Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mbcAT/RES-TIGR02293
Location 2225059..2225957 Replicon chromosome
Accession NZ_CP089780
Organism Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)


Gene name mbcT Uniprot ID P64908
Locus tag LVJ75_RS10420 Protein ID WP_003410001.1
Coordinates 2225059..2225619 (-) Length 187 a.a.

Antitoxin (Protein)


Gene name mbcA Uniprot ID P64910
Locus tag LVJ75_RS10425 Protein ID WP_003410003.1
Coordinates 2225616..2225957 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ75_RS10390 (LVJ75_10385) 2220228..2220881 - 654 WP_003409976.1 cutinase Cfp21 -
LVJ75_RS10395 (LVJ75_10390) 2220996..2221226 + 231 WP_003409980.1 DUF167 domain-containing protein -
LVJ75_RS10400 (LVJ75_10395) 2221311..2222222 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
LVJ75_RS10405 (LVJ75_10400) 2222331..2222930 + 600 WP_003910883.1 L-lysine exporter -
LVJ75_RS10410 (LVJ75_10405) 2223346..2223774 + 429 WP_003409992.1 cellulose-binding protein -
LVJ75_RS10415 (LVJ75_10410) 2224000..2224539 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
LVJ75_RS10420 (LVJ75_10415) 2225059..2225619 - 561 WP_003410001.1 RES family NAD+ phosphorylase Toxin
LVJ75_RS10425 (LVJ75_10420) 2225616..2225957 - 342 WP_003410003.1 DUF2384 domain-containing protein Antitoxin
LVJ75_RS10430 (LVJ75_10425) 2226043..2226300 + 258 WP_003410006.1 hypothetical protein -
LVJ75_RS10435 (LVJ75_10430) 2226201..2226536 - 336 WP_003410009.1 dehydrogenase -
LVJ75_RS10440 (LVJ75_10435) 2226625..2226969 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 -
LVJ75_RS10445 (LVJ75_10440) 2226963..2227211 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family -
LVJ75_RS10450 (LVJ75_10445) 2227311..2229626 - 2316 WP_003899120.1 cation transporter ATPase CptG -
LVJ75_RS10455 (LVJ75_10450) 2229623..2229895 - 273 WP_003410017.1 DUF1490 family protein -
LVJ75_RS10460 (LVJ75_10455) 2229948..2230304 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(25-148)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(69-110)


Sequences

Toxin        


Download         Length: 187 a.a.        Molecular weight: 20248.70 Da        Isoelectric Point: 4.5091

>T229178 WP_003410001.1 NZ_CP089780:c2225619-2225059 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Download         Length: 561 bp

>T229178 NZ_CP124410:c2500613-2500506 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12488.33 Da        Isoelectric Point: 4.8359

>AT229178 WP_003410003.1 NZ_CP089780:c2225957-2225616 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Download         Length: 342 bp

>AT229178 NZ_CP124410:2500662-2500727 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FKG

Antitoxin

Source ID Structure
PDB 6FKG
AlphaFold DB A0A7U4FAE4

References