Detailed information of TA system TA229165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1238265..1238833
• Replicon: chromosome
• Accession: NZ_CP089780
• Organism: Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: LVJ75_RS05930
• Protein ID: WP_003405865.1
• Coordinates: 1238459..1238833 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: LVJ75_RS05925
• Protein ID: WP_003405863.1
• Coordinates: 1238265..1238462 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ75_RS05905 (LVJ75_05895)	1234306..1234944	-	639	WP_003898730.1	lipid droplet-associated protein	-
LVJ75_RS05910 (LVJ75_05900)	1235034..1236041	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LVJ75_RS05915 (LVJ75_05905)	1236058..1237041	-	984	WP_003898731.1	hypothetical protein	-
LVJ75_RS05920 (LVJ75_05910)	1237104..1238177	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
LVJ75_RS05925 (LVJ75_05915)	1238265..1238462	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ75_RS05930 (LVJ75_05920)	1238459..1238833	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ75_RS05935 (LVJ75_05925)	1239036..1239734	+	699	WP_003898733.1	hypothetical protein	-
LVJ75_RS05940 (LVJ75_05930)	1239852..1240037	+	186	WP_003901093.1	hypothetical protein	-
LVJ75_RS05945 (LVJ75_05935)	1239964..1240239	-	276	WP_003405867.1	hypothetical protein	-
LVJ75_RS05950 (LVJ75_05940)	1240482..1240805	+	324	WP_003405871.1	putative quinol monooxygenase	-
LVJ75_RS05955 (LVJ75_05945)	1240820..1241518	-	699	WP_031646953.1	hypothetical protein	-
LVJ75_RS05960 (LVJ75_05950)	1241552..1241761	+	210	WP_003911400.1	hypothetical protein	-
LVJ75_RS05965 (LVJ75_05955)	1241713..1242483	-	771	Protein_1175	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T229165 WP_003405865.1 NZ_CP089780:1238459-1238833 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T229165 NZ_CP124406:82830-83210 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT229165 WP_003405863.1 NZ_CP089780:1238265-1238462 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT229165 NZ_CP124406:82609-82830 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07