229164

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1229509..1230140 Replicon chromosome
Accession NZ_CP089780
Organism Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag LVJ75_RS05875 Protein ID WP_003405820.1
Coordinates 1229509..1229820 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag LVJ75_RS05880 Protein ID WP_003405836.1
Coordinates 1229820..1230140 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ75_RS05855 (LVJ75_05845) 1224990..1226414 - 1425 WP_003405805.1 class II fumarate hydratase -
LVJ75_RS05860 (LVJ75_05850) 1226445..1227533 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
LVJ75_RS05865 (LVJ75_05855) 1227589..1228233 + 645 WP_003915494.1 DUF4245 domain-containing protein -
LVJ75_RS05870 (LVJ75_05860) 1228240..1229397 - 1158 WP_003898727.1 AI-2E family transporter -
LVJ75_RS05875 (LVJ75_05865) 1229509..1229820 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
LVJ75_RS05880 (LVJ75_05870) 1229820..1230140 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
LVJ75_RS05885 (LVJ75_05875) 1230150..1231686 + 1537 Protein_1159 carboxylesterase/lipase family protein -
LVJ75_RS05890 (LVJ75_05880) 1231693..1232805 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
LVJ75_RS05895 (LVJ75_05885) 1232815..1233072 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
LVJ75_RS05900 (LVJ75_05890) 1233062..1234309 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
LVJ75_RS05905 (LVJ75_05895) 1234306..1234944 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T229164 WP_003405820.1 NZ_CP089780:c1229820-1229509 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T229164 NZ_CP124406:c72457-72152 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT229164 WP_003405836.1 NZ_CP089780:c1230140-1229820 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT229164 NZ_CP124406:c72677-72459 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References