229163

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1022894..1023867 Replicon chromosome
Accession NZ_CP089780
Organism Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)


Gene name tacT Uniprot ID O05911
Locus tag LVJ75_RS04855 Protein ID WP_003404756.1
Coordinates 1023367..1023867 (+) Length 167 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID G0TFR4
Locus tag LVJ75_RS04850 Protein ID WP_003404754.1
Coordinates 1022894..1023370 (+) Length 159 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ75_RS04830 (LVJ75_04820) 1018741..1020012 - 1272 WP_003404747.1 PPE family protein -
LVJ75_RS04835 (LVJ75_04825) 1020027..1020326 - 300 WP_003404748.1 PE family protein -
LVJ75_RS04840 (LVJ75_04830) 1020770..1022551 + 1782 WP_003404749.1 BCCT family transporter -
LVJ75_RS04845 (LVJ75_04835) 1022551..1022805 + 255 WP_003898646.1 hypothetical protein -
LVJ75_RS04850 (LVJ75_04840) 1022894..1023370 + 477 WP_003404754.1 DUF1778 domain-containing protein Antitoxin
LVJ75_RS04855 (LVJ75_04845) 1023367..1023867 + 501 WP_003404756.1 GNAT family N-acetyltransferase Toxin
LVJ75_RS04865 (LVJ75_04855) 1024180..1025499 - 1320 WP_003404757.1 IS256-like element IS1554 family transposase -
LVJ75_RS04870 (LVJ75_04860) 1025787..1026368 + 582 WP_003404760.1 IS607-like element IS1535 family transposase -
LVJ75_RS04875 (LVJ75_04865) 1026368..1028020 + 1653 WP_003905412.1 IS607 family element RNA-guided endonuclease TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(38-143)

Antitoxin

Vibrio phage ICP1, Orf50

(74-150)


Sequences

Toxin        


Download         Length: 167 a.a.        Molecular weight: 18048.55 Da        Isoelectric Point: 7.8659

>T229163 WP_003404756.1 NZ_CP089780:1023367-1023867 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Download         Length: 501 bp

>T229163 NZ_CP124406:c53877-53743 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin


Download         Length: 159 a.a.        Molecular weight: 17357.95 Da        Isoelectric Point: 11.2467

>AT229163 WP_003404754.1 NZ_CP089780:1022894-1023370 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGGSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Download         Length: 477 bp

>AT229163 NZ_CP124406:53922-53982 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSZ2

Antitoxin

Source ID Structure
AlphaFold DB G0TFR4

References