Detailed information of TA system TA022916

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): brnT-shpB/BrnT-BrnA
• Location: 2369261..2369858
• Replicon: chromosome
• Accession: NC_010814
• Organism: Geobacter lovleyi SZ

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: B3E4B6
• Locus tag: GLOV_RS11015
• Protein ID: WP_012470273.1
• Coordinates: 2369565..2369858 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B3E4B5
• Locus tag: GLOV_RS11010
• Protein ID: WP_012470272.1
• Coordinates: 2369261..2369581 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GLOV_RS10985	2364863..2365150	+	288	WP_012470267.1	HU family DNA-binding protein	-
GLOV_RS10990	2365234..2366340	-	1107	WP_012470268.1	NAD(P)/FAD-dependent oxidoreductase	-
GLOV_RS10995	2366330..2367043	-	714	WP_041243347.1	GMP/IMP nucleotidase	-
GLOV_RS11000	2367128..2368855	-	1728	WP_012470270.1	DUF3373 domain-containing protein	-
GLOV_RS11005	2369033..2369257	-	225	WP_012470271.1	DUF1450 domain-containing protein	-
GLOV_RS11010	2369261..2369581	-	321	WP_012470272.1	BrnA antitoxin family protein	Antitoxin
GLOV_RS11015	2369565..2369858	-	294	WP_012470273.1	BrnT family toxin	Toxin
GLOV_RS11020	2369912..2370442	-	531	WP_012470274.1	ferritin	-
GLOV_RS11025	2370579..2374229	+	3651	WP_012470275.1	DUF748 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (8-89)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (42-96)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11237.82 Da
• Isoelectric Point: 6.8809

>T22916 WP_012470273.1 NC_010814:c2369858-2369565 [Geobacter lovleyi SZ]
MSSWLFEWDEIKNKSNKLKHGVSFETAAHVFNDPLLLSVQDRHIDGEERWQSIGMVNGNVLLLVAHLFQEDAKVVVIRIV
SARKATAYERRRYEEGI

Nucleotide

• Length: 294 bp

>T22916 NC_010814:c2369858-2369565 [Geobacter lovleyi SZ]
ATGAGCTCATGGTTGTTTGAGTGGGATGAAATCAAAAACAAGTCCAATAAGCTGAAACATGGTGTTAGCTTTGAAACCGC
TGCCCATGTCTTTAACGATCCGCTATTGTTGAGTGTGCAGGATCGTCACATAGATGGGGAAGAACGTTGGCAGTCAATTG
GTATGGTGAACGGAAATGTTTTGCTGCTGGTTGCCCACCTGTTTCAAGAAGATGCTAAGGTGGTCGTGATTCGTATTGTT
TCAGCGCGAAAAGCGACAGCTTACGAAAGAAGGCGTTATGAAGAAGGGATCTGA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11814.52 Da
• Isoelectric Point: 10.1356

>AT22916 WP_012470272.1 NC_010814:c2369581-2369261 [Geobacter lovleyi SZ]
MKKGSEKQTVVRKQSVAELTAEQLDELKNLASLPDTKIDCSDAPELTNWNEAVIGKFYRPVKQTITIRLDADVVSWLKTG
GKGYQSRANAILRQVMNQQGQPRKAA

Nucleotide

• Length: 321 bp

>AT22916 NC_010814:c2369581-2369261 [Geobacter lovleyi SZ]
ATGAAGAAGGGATCTGAAAAACAGACGGTCGTGCGTAAGCAGTCAGTTGCTGAATTGACGGCAGAACAACTGGATGAATT
AAAAAACTTGGCCAGTTTACCTGATACCAAGATTGATTGTTCAGACGCTCCAGAGCTTACTAATTGGAATGAGGCGGTGA
TTGGCAAGTTTTATCGTCCTGTAAAACAGACCATCACCATCCGCCTTGATGCAGATGTCGTGTCTTGGTTAAAGACCGGC
GGCAAAGGCTATCAAAGCCGTGCTAATGCAATTCTGCGCCAGGTTATGAATCAGCAAGGTCAGCCTCGTAAGGCTGCATA
A

Structures

Toxin

• AlphaFold DB: B3E4B6

Antitoxin

• AlphaFold DB: B3E4B5