229159

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapC-mazE/PIN(toxin)
Location 753965..754757 Replicon chromosome
Accession NZ_CP089780
Organism Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB2
Locus tag LVJ75_RS03475 Protein ID WP_003403386.1
Coordinates 754320..754757 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag LVJ75_RS03470 Protein ID WP_003403381.1
Coordinates 753965..754210 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ75_RS03440 (LVJ75_03435) 748985..750490 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
LVJ75_RS03445 (LVJ75_03440) 750571..751581 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
LVJ75_RS03450 (LVJ75_03445) 751969..752352 - 384 WP_003403365.1 ribonuclease VapC6 -
LVJ75_RS03455 (LVJ75_03450) 752447..752602 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ75_RS03460 (LVJ75_03455) 752678..753394 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
LVJ75_RS03465 (LVJ75_03460) 753670..753978 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
LVJ75_RS03470 (LVJ75_03465) 753965..754210 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
LVJ75_RS03475 (LVJ75_03470) 754320..754757 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ75_RS03480 (LVJ75_03475) 754754..755008 - 255 WP_003911263.1 antitoxin VapB7 -
LVJ75_RS03485 (LVJ75_03480) 755122..757485 + 2364 WP_003901895.1 arylsulfatase AtsD -
LVJ75_RS03490 (LVJ75_03485) 757548..757874 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ75_RS03495 (LVJ75_03490) 757786..758124 + 339 WP_003403405.1 PIN domain-containing protein -
LVJ75_RS03500 (LVJ75_03495) 758121..758294 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-126)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15245.39 Da        Isoelectric Point: 5.1865

>T229159 WP_003403386.1 NZ_CP089780:c754757-754320 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Download         Length: 438 bp

>T229159 NZ_CP124405:3826507-3827427 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCAGGCAGGTTAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAATCCTGTTT
CGCGTTGAAAGGCGGCACAGCAATCAATTTATTTGTGCAGGATTTCCCCCGTCTATCGGTGGATATCGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACCGATCTGGCGGCGATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATATGTGCTGCGCTTGATCGTCAGCAT
CCGCGCGATCTGTTTGATGTGTTGAATATGCTGGGGAGACCGGGCCTGACGCGCGAAATTTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGTCCCATTGCCGAGTTGCTGGCTTCAAACTGGGATACAGATCGGATTGCCACGTTATACGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATTTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGATGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGAGTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT229159 WP_003403381.1 NZ_CP089780:c754210-753965 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT229159 NZ_CP124405:3825738-3826520 [Escherichia coli]
TTGCCAAACTATCTAAACTGGTTGATGCAAAACACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAATGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAGAATGGCTGGCTCAGGCGCATCGCTCACGGTGTGTACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTTCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGCCATGCGCACTATACCGAACCGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTGCTGCCTTCGAACAATCAGCAACATTTATCACGCTGTATACATCGGGCCTTACCGTTGATGTCT
CGTCATTTACTACGGAACTGAAAATTGGCGACCGGCAGTTAGCAGGCAGTGCACCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGGGGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGATTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATCTTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCTGGTGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCCCGCACATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSW5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References