Detailed information of TA system TA229157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 751969..752602
• Replicon: chromosome
• Accession: NZ_CP089780
• Organism: Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQD6
• Locus tag: LVJ75_RS03450
• Protein ID: WP_003403365.1
• Coordinates: 751969..752352 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: LVJ75_RS03455
• Protein ID: WP_003403368.1
• Coordinates: 752447..752602 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ75_RS03425 (LVJ75_03420)	747261..747797	+	537	WP_003403341.1	50S ribosomal protein L10	-
LVJ75_RS03430 (LVJ75_03425)	747834..748226	+	393	WP_003403353.1	50S ribosomal protein L7/L12	-
LVJ75_RS03435 (LVJ75_03430)	748219..748914	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
LVJ75_RS03440 (LVJ75_03435)	748985..750490	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
LVJ75_RS03445 (LVJ75_03440)	750571..751581	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
LVJ75_RS03450 (LVJ75_03445)	751969..752352	-	384	WP_003403365.1	ribonuclease VapC6	Toxin
LVJ75_RS03455 (LVJ75_03450)	752447..752602	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ75_RS03460 (LVJ75_03455)	752678..753394	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
LVJ75_RS03465 (LVJ75_03460)	753670..753978	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
LVJ75_RS03470 (LVJ75_03465)	753965..754210	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
LVJ75_RS03475 (LVJ75_03470)	754320..754757	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
LVJ75_RS03480 (LVJ75_03475)	754754..755008	-	255	WP_003911263.1	antitoxin VapB7	-
LVJ75_RS03485 (LVJ75_03480)	755122..757485	+	2364	WP_003901895.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13959.73 Da
• Isoelectric Point: 6.0803

>T229157 WP_003403365.1 NZ_CP089780:c752352-751969 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T229157 NZ_CP124405:3446740-3447027 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT229157 WP_003403368.1 NZ_CP089780:c752602-752447 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT229157 NZ_CP124405:3447014-3447316 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: G0TQD6

Antitoxin

• AlphaFold DB: A0A7U4BSF8