Detailed information of TA system TA229155

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 714842..715491
• Replicon: chromosome
• Accession: NZ_CP089780
• Organism: Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67241
• Locus tag: LVJ75_RS03265
• Protein ID: WP_003403236.1
• Coordinates: 715096..715491 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ34
• Locus tag: LVJ75_RS03260
• Protein ID: WP_003403235.1
• Coordinates: 714842..715096 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ75_RS03235 (LVJ75_03230)	709968..710051	+	84	Protein_641	galactose-1-phosphate uridylyltransferase	-
LVJ75_RS03240 (LVJ75_03235)	710070..711151	+	1082	Protein_642	galactose-1-phosphate uridylyltransferase	-
LVJ75_RS03245 (LVJ75_03240)	711148..712239	+	1092	WP_003900977.1	galactokinase	-
LVJ75_RS03250 (LVJ75_03245)	712634..713698	+	1065	WP_003898532.1	zinc ribbon domain-containing protein	-
LVJ75_RS03255 (LVJ75_03250)	713802..714749	+	948	WP_003403232.1	DUF732 domain-containing protein	-
LVJ75_RS03260 (LVJ75_03255)	714842..715096	+	255	WP_003403235.1	type II toxin-antitoxin system antitoxin VapB30	Antitoxin
LVJ75_RS03265 (LVJ75_03260)	715096..715491	+	396	WP_003403236.1	type II toxin-antitoxin system toxin ribonuclease C30	Toxin
LVJ75_RS03270 (LVJ75_03265)	715585..716325	-	741	WP_003403239.1	TVP38/TMEM64 family protein	-
LVJ75_RS03275 (LVJ75_03270)	716457..716717	+	261	WP_003403244.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LVJ75_RS03280 (LVJ75_03275)	716714..717121	+	408	WP_003403246.1	type II toxin-antitoxin system VapC family toxin	-
LVJ75_RS03285 (LVJ75_03280)	717193..718344	-	1152	WP_003403248.1	FIST N-terminal domain-containing protein	-
LVJ75_RS03290 (LVJ75_03285)	718437..720164	-	1728	WP_003901883.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14250.04 Da
• Isoelectric Point: 4.7475

>T229155 WP_003403236.1 NZ_CP089780:715096-715491 [Mycobacterium tuberculosis]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Nucleotide

• Length: 396 bp

>T229155 NZ_CP124405:3265265-3265615 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9139.52 Da
• Isoelectric Point: 9.2702

>AT229155 WP_003403235.1 NZ_CP089780:714842-715096 [Mycobacterium tuberculosis]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Nucleotide

• Length: 255 bp

>AT229155 NZ_CP124405:3265020-3265271 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• PDB: 4XGR
• PDB: 4XGQ

Antitoxin

• AlphaFold DB: A0A7U4BSC2