Detailed information of TA system TA229149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 638664..639340
• Replicon: chromosome
• Accession: NZ_CP089780
• Organism: Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: LVJ75_RS02875
• Protein ID: WP_003898500.1
• Coordinates: 638664..639077 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: LVJ75_RS02880
• Protein ID: WP_003402915.1
• Coordinates: 639074..639340 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ75_RS02845 (LVJ75_02840)	634009..634311	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
LVJ75_RS02850 (LVJ75_02845)	634371..634649	-	279	WP_003402901.1	hypothetical protein	-
LVJ75_RS02855 (LVJ75_02850)	634646..635899	-	1254	WP_003402904.1	inorganic phosphate transporter	-
LVJ75_RS02860 (LVJ75_02855)	636019..636405	-	387	WP_003402907.1	VOC family protein	-
LVJ75_RS02865 (LVJ75_02860)	636468..637352	-	885	WP_003402909.1	SDR family oxidoreductase	-
LVJ75_RS02870 (LVJ75_02865)	637448..638350	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
LVJ75_RS02875 (LVJ75_02870)	638664..639077	-	414	WP_003898500.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ75_RS02880 (LVJ75_02875)	639074..639340	-	267	WP_003402915.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LVJ75_RS02885 (LVJ75_02880)	639532..641247	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
LVJ75_RS02890 (LVJ75_02885)	641325..642929	+	1605	WP_003402920.1	amidohydrolase	-
LVJ75_RS02895 (LVJ75_02890)	642926..643906	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T229149 WP_003898500.1 NZ_CP089780:c639077-638664 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T229149 NZ_CP124405:1997097-1997561 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT229149 WP_003402915.1 NZ_CP089780:c639340-639074 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT229149 NZ_CP124405:1996762-1997097 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0