Detailed information of TA system TA229148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 442599..443610
• Replicon: chromosome
• Accession: NZ_CP089780
• Organism: Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: L7N5R2
• Locus tag: LVJ75_RS01915
• Protein ID: WP_003401859.1
• Coordinates: 442599..443192 (-)
• Length: 198 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: O53702
• Locus tag: LVJ75_RS01920
• Protein ID: WP_003401860.1
• Coordinates: 443221..443610 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ75_RS01895 (LVJ75_01895)	438248..439630	+	1383	WP_003898415.1	magnesium transporter	-
LVJ75_RS01900 (LVJ75_01900)	439642..440676	-	1035	WP_003401856.1	class II fructose-bisphosphate aldolase	-
LVJ75_RS01905 (LVJ75_01905)	440772..441455	+	684	WP_003401857.1	VTT domain-containing protein	-
LVJ75_RS01910 (LVJ75_01910)	441444..442574	-	1131	WP_003401858.1	glycoside hydrolase family 76 protein	-
LVJ75_RS01915 (LVJ75_01915)	442599..443192	-	594	WP_003401859.1	zeta toxin family protein	Toxin
LVJ75_RS01920 (LVJ75_01920)	443221..443610	-	390	WP_003401860.1	hypothetical protein	Antitoxin
LVJ75_RS01925 (LVJ75_01925)	443691..444902	-	1212	WP_003401861.1	VWA domain-containing protein	-
LVJ75_RS01930 (LVJ75_01930)	444908..445510	-	603	WP_003898417.1	SRPBCC family protein	-
LVJ75_RS01935 (LVJ75_01935)	445524..446420	-	897	WP_003898418.1	MoxR family ATPase	-
LVJ75_RS01940 (LVJ75_01940)	446417..447010	-	594	WP_003401864.1	NTP transferase domain-containing protein	-
LVJ75_RS01945 (LVJ75_01945)	447007..447762	-	756	WP_003401865.1	XdhC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-156)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 198 a.a.
• Molecular weight: 21344.53 Da
• Isoelectric Point: 10.5018

>T229148 WP_003401859.1 NZ_CP089780:c443192-442599 [Mycobacterium tuberculosis]
VKRLDLVAGPNGAGKSTFVALTLAPLLPGIVFVNADEIAKQRWPDDPTSHAYQAAQVAADTRARLIDLGRPFIAETVFSH
PSKLELIRTARTAGYTVVLHVLVIPEGLAVERVRHRVAAGGHDVPETKIRERHRRLAELVAQAITLADGATVYDNSRLAG
PRIVAQFSGGGIIGRACWPSWTPPPLMSRWSNRPETA

Nucleotide

• Length: 594 bp

>T229148 NZ_CP124405:c1955781-1955467 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13648.21 Da
• Isoelectric Point: 6.0829

>AT229148 WP_003401860.1 NZ_CP089780:c443610-443221 [Mycobacterium tuberculosis]
VPKAVDRVTRVAADLVDSAAAEGARQSRSAKQQLDHWARVGRAVSNQHTASRRRVEAALAGHLPMTDLTLEEGVVFNAEI
SAAIEERLSRTNYGDVLAAQGITTVALNDAGDIVEHRPDGTSVVLAATP

Nucleotide

• Length: 390 bp

>AT229148 NZ_CP124405:c1955470-1955054 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A7U4BRU8

Antitoxin

• AlphaFold DB: A0A7U4BRT0