Detailed information of TA system TA229146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 361937..362508
• Replicon: chromosome
• Accession: NZ_CP089780
• Organism: Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: LVJ75_RS01570
• Protein ID: WP_003401560.1
• Coordinates: 361937..362239 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: LVJ75_RS01575
• Protein ID: WP_003401563.1
• Coordinates: 362287..362508 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ75_RS01550 (LVJ75_01550)	357406..358209	-	804	WP_003401540.1	Stf0 family sulphotransferase	-
LVJ75_RS01555 (LVJ75_01555)	358219..359616	-	1398	WP_003401544.1	sulfatase	-
LVJ75_RS01560 (LVJ75_01560)	359795..361570	+	1776	WP_010886074.1	PE family protein	-
LVJ75_RS01565 (LVJ75_01565)	361713..361940	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
LVJ75_RS01570 (LVJ75_01570)	361937..362239	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
LVJ75_RS01575 (LVJ75_01575)	362287..362508	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
LVJ75_RS01580 (LVJ75_01580)	362505..362930	+	426	WP_003401566.1	PIN domain nuclease	-
LVJ75_RS01585 (LVJ75_01585)	363066..363698	+	633	WP_003905275.1	TetR/AcrR family transcriptional regulator	-
LVJ75_RS01590 (LVJ75_01590)	363695..364603	+	909	WP_003900117.1	protochlorophyllide reductase	-
LVJ75_RS01595 (LVJ75_01595)	364611..365201	-	591	WP_229298012.1	hypothetical protein	-
LVJ75_RS01600 (LVJ75_01600)	365194..365244	-	51	Protein_316	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T229146 WP_003401560.1 NZ_CP089780:361937-362239 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T229146 NZ_CP124405:c789616-789365 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT229146 WP_003401563.1 NZ_CP089780:362287-362508 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT229146 NZ_CP124405:c789368-789114 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CG48