Detailed information of TA system TA229141

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 3891895..3892466
• Replicon: chromosome
• Accession: NZ_CP089779
• Organism: Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: LVJ74_RS18400
• Protein ID: WP_003401560.1
• Coordinates: 3892164..3892466 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: LVJ74_RS18395
• Protein ID: WP_003401563.1
• Coordinates: 3891895..3892116 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ74_RS18380 (LVJ74_18370)	3889800..3890708	-	909	WP_003900117.1	protochlorophyllide reductase	-
LVJ74_RS18385 (LVJ74_18375)	3890705..3891337	-	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
LVJ74_RS18390 (LVJ74_18380)	3891473..3891898	-	426	WP_003401566.1	PIN domain nuclease	-
LVJ74_RS18395 (LVJ74_18385)	3891895..3892116	-	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
LVJ74_RS18400 (LVJ74_18390)	3892164..3892466	-	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
LVJ74_RS18405 (LVJ74_18395)	3892463..3892690	-	228	WP_031658821.1	ribbon-helix-helix protein, CopG family	-
LVJ74_RS18410 (LVJ74_18400)	3892833..3894572	-	1740	WP_031658820.1	PE family protein	-
LVJ74_RS18415 (LVJ74_18405)	3894751..3896148	+	1398	WP_003401544.1	sulfatase	-
LVJ74_RS18420 (LVJ74_18410)	3896158..3896961	+	804	WP_003401540.1	Stf0 family sulphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T229141 WP_003401560.1 NZ_CP089779:c3892466-3892164 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T229141 NZ_CP124401:89032-89412 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT229141 WP_003401563.1 NZ_CP089779:c3892116-3891895 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT229141 NZ_CP124401:88811-89032 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CG48