Detailed information of TA system TA022914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 1270711..1271307
• Replicon: chromosome
• Accession: NC_010814
• Organism: Geobacter lovleyi SZ

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B3E763
• Locus tag: GLOV_RS06005
• Protein ID: WP_012469291.1
• Coordinates: 1270711..1271001 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: GLOV_RS06010
• Protein ID: WP_012469292.1
• Coordinates: 1271011..1271307 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GLOV_RS06005	1270711..1271001	+	291	WP_012469291.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GLOV_RS06010	1271011..1271307	+	297	WP_012469292.1	putative addiction module antidote protein	Antitoxin
GLOV_RS06015	1271657..1272358	-	702	WP_012469293.1	hypothetical protein	-
GLOV_RS06020	1272691..1273116	-	426	WP_012469294.1	PIN domain-containing protein	-
GLOV_RS06025	1273113..1273355	-	243	WP_012469295.1	hypothetical protein	-
GLOV_RS06030	1273431..1274021	-	591	WP_012469296.1	AMMECR1 domain-containing protein	-
GLOV_RS06035	1274018..1274770	-	753	WP_153304653.1	VanW family protein	-
GLOV_RS06040	1274831..1275973	-	1143	WP_012469298.1	radical SAM family heme chaperone HemW	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-92)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10771.45 Da
• Isoelectric Point: 10.4470

>T22914 WP_012469291.1 NC_010814:1270711-1271001 [Geobacter lovleyi SZ]
MIEIRKTEQFANWLDNLRDIQAKARVLVRIERLASGNAGDVKPVGEGISEMRINYGPGYRVYFVQRGSELIILLAGGDKS
NQSRDIKAAIRLAQKL

Nucleotide

• Length: 291 bp

>T22914 NC_010814:1270711-1271001 [Geobacter lovleyi SZ]
ATGATTGAAATCCGTAAAACTGAACAATTTGCCAACTGGCTTGACAACCTGCGTGATATCCAAGCCAAGGCTCGCGTCTT
GGTCAGGATTGAACGTCTTGCCTCGGGAAATGCCGGCGACGTCAAGCCTGTTGGCGAAGGTATTTCGGAAATGAGAATCA
ACTACGGTCCCGGTTATCGTGTCTATTTTGTCCAACGCGGCAGCGAGTTGATCATCCTGCTGGCTGGTGGTGACAAGAGC
AACCAATCTCGGGATATCAAGGCAGCAATTCGCCTTGCACAGAAATTATAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10503.91 Da
• Isoelectric Point: 6.2258

>AT22914 WP_012469292.1 NC_010814:1271011-1271307 [Geobacter lovleyi SZ]
MTRTTTSRYDVAEHLRTPEEMAAYLEASLEEANGDAAFIAKALGDIARAKGMSQVARDAGLSRESLYKALSGERVPGFDT
ILKIVSALGLKLHAEAAH

Nucleotide

• Length: 297 bp

>AT22914 NC_010814:1271011-1271307 [Geobacter lovleyi SZ]
ATGACCAGAACAACTACCAGTCGTTATGATGTTGCCGAGCATCTCCGTACTCCGGAAGAAATGGCAGCCTATCTTGAAGC
TTCCCTTGAAGAGGCCAATGGCGATGCCGCTTTTATTGCCAAGGCCCTCGGTGATATTGCCCGTGCCAAGGGGATGTCGC
AGGTGGCTCGTGATGCCGGTCTTTCCCGTGAAAGTCTTTATAAGGCCCTCTCCGGAGAAAGAGTTCCAGGTTTTGATACA
ATTCTCAAAATTGTTTCGGCTTTGGGACTCAAACTTCACGCTGAAGCTGCCCATTAA

Structures

Toxin

• AlphaFold DB: B3E763