Detailed information of TA system TA229136

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 3560559..3561205
• Replicon: chromosome
• Accession: NZ_CP089779
• Organism: Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: O07783
• Locus tag: LVJ74_RS16870
• Protein ID: WP_003403122.1
• Coordinates: 3560813..3561205 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ86
• Locus tag: LVJ74_RS16865
• Protein ID: WP_003403125.1
• Coordinates: 3560559..3560816 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ74_RS16830 (LVJ74_16820)	3555877..3556188	-	312	WP_003403164.1	hypothetical protein	-
LVJ74_RS16835 (LVJ74_16825)	3556311..3557006	+	696	WP_003918099.1	two-component system response regulator TcrA	-
LVJ74_RS16840 (LVJ74_16830)	3556990..3557520	+	531	WP_003917320.1	two component system sensor kinase HK2	-
LVJ74_RS16845 (LVJ74_16835)	3557634..3558140	+	507	WP_003900973.1	two component system sensor kinase HK1	-
LVJ74_RS16850 (LVJ74_16840)	3558244..3558480	+	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	-
LVJ74_RS16855 (LVJ74_16845)	3558477..3558890	+	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	-
LVJ74_RS16860 (LVJ74_16850)	3559141..3560376	+	1236	WP_003403128.1	ATP-binding protein	-
LVJ74_RS16865 (LVJ74_16855)	3560559..3560816	+	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	Antitoxin
LVJ74_RS16870 (LVJ74_16860)	3560813..3561205	+	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	Toxin
LVJ74_RS16875 (LVJ74_16865)	3561257..3562807	-	1551	WP_003403119.1	MlaD family protein	-
LVJ74_RS16880 (LVJ74_16870)	3562812..3563954	-	1143	WP_003911253.1	virulence factor Mce family protein	-
LVJ74_RS16885 (LVJ74_16875)	3564017..3565543	-	1527	WP_003403112.1	virulence factor Mce family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14080.08 Da
• Isoelectric Point: 4.6558

>T229136 WP_003403122.1 NZ_CP089779:3560813-3561205 [Mycobacterium tuberculosis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Nucleotide

• Length: 393 bp

>T229136 NZ_CP124400:c4061561-4061343 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9681.06 Da
• Isoelectric Point: 7.0175

>AT229136 WP_003403125.1 NZ_CP089779:3560559-3560816 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Nucleotide

• Length: 258 bp

>AT229136 NZ_CP124400:c4061961-4061587 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7U4F8V4

Antitoxin

• AlphaFold DB: G0TQ86