Detailed information of TA system TA229135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 3558244..3558890
• Replicon: chromosome
• Accession: NZ_CP089779
• Organism: Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: LVJ74_RS16855
• Protein ID: WP_003403137.1
• Coordinates: 3558477..3558890 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: LVJ74_RS16850
• Protein ID: WP_003403139.1
• Coordinates: 3558244..3558480 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ74_RS16815 (LVJ74_16805)	3553318..3554028	-	711	Protein_3316	IS607 family element RNA-guided endonuclease TnpB	-
LVJ74_RS16820 (LVJ74_16810)	3554030..3554614	-	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
LVJ74_RS16825 (LVJ74_16815)	3554855..3555805	-	951	WP_003900184.1	DUF1259 domain-containing protein	-
LVJ74_RS16830 (LVJ74_16820)	3555877..3556188	-	312	WP_003403164.1	hypothetical protein	-
LVJ74_RS16835 (LVJ74_16825)	3556311..3557006	+	696	WP_003918099.1	two-component system response regulator TcrA	-
LVJ74_RS16840 (LVJ74_16830)	3556990..3557520	+	531	WP_003917320.1	two component system sensor kinase HK2	-
LVJ74_RS16845 (LVJ74_16835)	3557634..3558140	+	507	WP_003900973.1	two component system sensor kinase HK1	-
LVJ74_RS16850 (LVJ74_16840)	3558244..3558480	+	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
LVJ74_RS16855 (LVJ74_16845)	3558477..3558890	+	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ74_RS16860 (LVJ74_16850)	3559141..3560376	+	1236	WP_003403128.1	ATP-binding protein	-
LVJ74_RS16865 (LVJ74_16855)	3560559..3560816	+	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
LVJ74_RS16870 (LVJ74_16860)	3560813..3561205	+	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
LVJ74_RS16875 (LVJ74_16865)	3561257..3562807	-	1551	WP_003403119.1	MlaD family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T229135 WP_003403137.1 NZ_CP089779:3558477-3558890 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T229135 NZ_CP124400:3876182-3877102 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCAGGCAGGTTAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAATCCTGTTT
CGCGTTGAAAGGCGGCACAGCAATCAATTTATTTGTGCAGGATTTCCCCCGTCTATCGGTGGATATCGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACCGATCTGGCGGCGATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATATGTGCTGCGCTTGATCGTCAGCAT
CCGCGCGATCTGTTTGATGTGTTGAATATGCTGGGGAGACCGGGCCTGACGCGCGAAATTTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGTCCCATTGCCGAGTTGCTGGCTTCAAACTGGGATACAGATCGGATTGCCACGTTATACGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATTTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGATGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGAGTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT229135 WP_003403139.1 NZ_CP089779:3558244-3558480 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT229135 NZ_CP124400:3875413-3876195 [Escherichia coli]
TTGCCAAACTATCTAAACTGGTTGATGCAAAACACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAATGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAGAATGGCTGGCTCAGGCGCATCGCTCACGGTGTGTACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTTCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGCCATGCGCACTATACCGAACCGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTGCTGCCTTCGAACAATCAGCAACATTTATCACGCTGTATACATCGGGCCTTACCGTTGATGTCT
CGTCATTTACTACGGAACTGAAAATTGGCGACCGGCAGTTAGCAGGCAGTGCACCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGGGGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGATTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATCTTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCTGGTGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCCCGCACATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89