Detailed information of TA system TA229134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 3552157..3552800
• Replicon: chromosome
• Accession: NZ_CP089779
• Organism: Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: LVJ74_RS16800
• Protein ID: WP_003403187.1
• Coordinates: 3552157..3552558 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: LVJ74_RS16805
• Protein ID: WP_003403184.1
• Coordinates: 3552555..3552800 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ74_RS16775 (LVJ74_16765)	3549115..3549720	-	606	WP_003898526.1	hypothetical protein	-
LVJ74_RS16780 (LVJ74_16770)	3549862..3550083	+	222	WP_003898525.1	DUF4926 domain-containing protein	-
LVJ74_RS16785 (LVJ74_16775)	3550135..3551292	+	1158	WP_003898524.1	hypothetical protein	-
LVJ74_RS16790 (LVJ74_16780)	3551372..3551569	+	198	WP_003403191.1	hypothetical protein	-
LVJ74_RS16795 (LVJ74_16785)	3551987..3552166	-	180	Protein_3312	hypothetical protein	-
LVJ74_RS16800 (LVJ74_16790)	3552157..3552558	-	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ74_RS16805 (LVJ74_16795)	3552555..3552800	-	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ74_RS16810 (LVJ74_16800)	3552845..3553315	-	471	WP_003898523.1	hypothetical protein	-
LVJ74_RS16815 (LVJ74_16805)	3553318..3554028	-	711	Protein_3316	IS607 family element RNA-guided endonuclease TnpB	-
LVJ74_RS16820 (LVJ74_16810)	3554030..3554614	-	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
LVJ74_RS16825 (LVJ74_16815)	3554855..3555805	-	951	WP_003900184.1	DUF1259 domain-containing protein	-
LVJ74_RS16830 (LVJ74_16820)	3555877..3556188	-	312	WP_003403164.1	hypothetical protein	-
LVJ74_RS16835 (LVJ74_16825)	3556311..3557006	+	696	WP_003918099.1	two-component system response regulator TcrA	-
LVJ74_RS16840 (LVJ74_16830)	3556990..3557520	+	531	WP_003917320.1	two component system sensor kinase HK2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T229134 WP_003403187.1 NZ_CP089779:c3552558-3552157 [Mycobacterium tuberculosis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T229134 NZ_CP124400:3609243-3609527 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT229134 WP_003403184.1 NZ_CP089779:c3552800-3552555 [Mycobacterium tuberculosis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT229134 NZ_CP124400:3609007-3609240 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91