Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 3537355..3538019 Replicon chromosome
Accession NZ_CP089779
Organism Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)


Gene name vapC Uniprot ID P96917
Locus tag LVJ74_RS16700 Protein ID WP_003403246.1
Coordinates 3537355..3537762 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WF18
Locus tag LVJ74_RS16705 Protein ID WP_003403244.1
Coordinates 3537759..3538019 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ74_RS16690 (LVJ74_16680) 3534312..3536039 + 1728 WP_003900979.1 exodeoxyribonuclease V subunit alpha -
LVJ74_RS16695 (LVJ74_16685) 3536132..3537283 + 1152 WP_003403248.1 FIST N-terminal domain-containing protein -
LVJ74_RS16700 (LVJ74_16690) 3537355..3537762 - 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ74_RS16705 (LVJ74_16695) 3537759..3538019 - 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LVJ74_RS16710 (LVJ74_16700) 3538151..3538891 + 741 WP_003403239.1 TVP38/TMEM64 family protein -
LVJ74_RS16715 (LVJ74_16705) 3538985..3539380 - 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 -
LVJ74_RS16720 (LVJ74_16710) 3539380..3539634 - 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 -
LVJ74_RS16725 (LVJ74_16715) 3539727..3540674 - 948 WP_003403232.1 DUF732 domain-containing protein -
LVJ74_RS16730 (LVJ74_16720) 3540778..3541842 - 1065 WP_003898532.1 zinc ribbon domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-125)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-57)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 14376.37 Da        Isoelectric Point: 4.3568

>T229131 WP_003403246.1 NZ_CP089779:c3537762-3537355 [Mycobacterium tuberculosis]
VSTTPAAGVLDTSVFIATESGRQLDEALIPDRVATTVVTLAELRVGVLAAATTDIRAQRLATLESVADMETLPVDDDAAR
MWARLRIHLAESGRRVRINDLWIAAVAASRALPVITQDDDFAALDGAASVEIIRV

Download         Length: 408 bp

>T229131 NZ_CP124400:3338929-3339279 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9492.84 Da        Isoelectric Point: 11.9108

>AT229131 WP_003403244.1 NZ_CP089779:c3538019-3537759 [Mycobacterium tuberculosis]
MSEVASRELRNDTAGVLRRVRAGEDVTITVSGRPVAVLTPVRPRRRRWLSKTEFLSRLRGAQADPGLRNDLAVLAGDTTE
DLGPIR

Download         Length: 261 bp

>AT229131 NZ_CP124400:3338684-3338935 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3DBO

Antitoxin

Source ID Structure
PDB 3DBO
AlphaFold DB A0A7U4BSE4

References