229129

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3500286..3500826 Replicon chromosome
Accession NZ_CP089779
Organism Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)


Gene name mazF Uniprot ID P9WII0
Locus tag LVJ74_RS16515 Protein ID WP_003403376.1
Coordinates 3500518..3500826 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag LVJ74_RS16510 Protein ID WP_003403381.1
Coordinates 3500286..3500531 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ74_RS16480 (LVJ74_16470) 3496202..3496375 - 174 WP_003898549.1 hypothetical protein -
LVJ74_RS16485 (LVJ74_16475) 3496372..3496710 - 339 WP_003403405.1 PIN domain-containing protein -
LVJ74_RS16490 (LVJ74_16480) 3496622..3496948 - 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ74_RS16495 (LVJ74_16485) 3497011..3499374 - 2364 WP_003898547.1 arylsulfatase AtsD -
LVJ74_RS16500 (LVJ74_16490) 3499488..3499742 + 255 WP_003911263.1 antitoxin VapB7 -
LVJ74_RS16505 (LVJ74_16495) 3499739..3500176 + 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
LVJ74_RS16510 (LVJ74_16500) 3500286..3500531 + 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
LVJ74_RS16515 (LVJ74_16505) 3500518..3500826 + 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LVJ74_RS16520 (LVJ74_16510) 3501102..3501818 + 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
LVJ74_RS16525 (LVJ74_16515) 3501894..3502049 + 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ74_RS16530 (LVJ74_16520) 3502144..3502527 + 384 WP_003403365.1 ribonuclease VapC6 -
LVJ74_RS16535 (LVJ74_16525) 3502915..3503925 - 1011 WP_003911248.1 ABC transporter ATP-binding protein -
LVJ74_RS16540 (LVJ74_16530) 3504006..3505511 - 1506 WP_003403360.1 carotenoid cleavage oxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-101)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11320.10 Da        Isoelectric Point: 11.6554

>T229129 WP_003403376.1 NZ_CP089779:3500518-3500826 [Mycobacterium tuberculosis]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Download         Length: 309 bp

>T229129 NZ_CP124400:2885103-2885414 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT229129 WP_003403381.1 NZ_CP089779:3500286-3500531 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT229129 NZ_CP124400:2885415-2885705 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSE5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References