Detailed information of TA system TA229125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 3240060..3240679
• Replicon: chromosome
• Accession: NZ_CP089779
• Organism: Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: LVJ74_RS15185
• Protein ID: WP_003404726.1
• Coordinates: 3240060..3240494 (-)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: LVJ74_RS15190
• Protein ID: WP_003898641.1
• Coordinates: 3240500..3240679 (-)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ74_RS15160 (LVJ74_15150)	3235395..3236633	+	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-
LVJ74_RS15165 (LVJ74_15155)	3236635..3238143	+	1509	WP_003404742.1	carotenoid oxygenase family protein	-
LVJ74_RS15170 (LVJ74_15160)	3238310..3238540	+	231	WP_003898642.1	hypothetical protein	-
LVJ74_RS15175 (LVJ74_15165)	3238675..3239124	-	450	WP_003404738.1	hypothetical protein	-
LVJ74_RS15180 (LVJ74_15170)	3239189..3239962	-	774	WP_003404735.1	VOC family protein	-
LVJ74_RS15185 (LVJ74_15175)	3240060..3240494	-	435	WP_003404726.1	SRPBCC family protein	Toxin
LVJ74_RS15190 (LVJ74_15180)	3240500..3240679	-	180	WP_003898641.1	antitoxin	Antitoxin
LVJ74_RS15195 (LVJ74_15185)	3240805..3240963	-	159	WP_003404720.1	hypothetical protein	-
LVJ74_RS15200 (LVJ74_15190)	3241236..3243629	-	2394	WP_003898639.1	cation-translocating P-type ATPase	-
LVJ74_RS15205 (LVJ74_15195)	3243626..3245206	-	1581	WP_003909313.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T229125 WP_003404726.1 NZ_CP089779:c3240494-3240060 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T229125 NZ_CP124400:2072740-2073204 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT229125 WP_003898641.1 NZ_CP089779:c3240679-3240500 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT229125 NZ_CP124400:2072405-2072740 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5