Detailed information of TA system TA229122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3024130..3024761
• Replicon: chromosome
• Accession: NZ_CP089779
• Organism: Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P9WIH8
• Locus tag: LVJ74_RS14130
• Protein ID: WP_003898728.1
• Coordinates: 3024450..3024761 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TGZ7
• Locus tag: LVJ74_RS14125
• Protein ID: WP_003405836.1
• Coordinates: 3024130..3024450 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ74_RS14100 (LVJ74_14090)	3019326..3019964	+	639	WP_003898730.1	lipid droplet-associated protein	-
LVJ74_RS14105 (LVJ74_14095)	3019961..3021208	+	1248	WP_003405846.1	exodeoxyribonuclease VII large subunit	-
LVJ74_RS14110 (LVJ74_14100)	3021198..3021455	+	258	WP_003405844.1	exodeoxyribonuclease VII small subunit	-
LVJ74_RS14115 (LVJ74_14105)	3021465..3022577	+	1113	WP_003405840.1	3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase	-
LVJ74_RS14120 (LVJ74_14110)	3022584..3024120	-	1537	Protein_2789	carboxylesterase/lipase family protein	-
LVJ74_RS14125 (LVJ74_14115)	3024130..3024450	+	321	WP_003405836.1	type II toxin-antitoxin system antitoxin MazE3	Antitoxin
LVJ74_RS14130 (LVJ74_14120)	3024450..3024761	+	312	WP_003898728.1	type II toxin-antitoxin system toxin endoribonuclease MazF3	Toxin
LVJ74_RS14135 (LVJ74_14125)	3024873..3026030	+	1158	WP_003898727.1	AI-2E family transporter	-
LVJ74_RS14140 (LVJ74_14130)	3026037..3026681	-	645	WP_003915494.1	DUF4245 domain-containing protein	-
LVJ74_RS14145 (LVJ74_14135)	3026737..3027825	+	1089	WP_003898726.1	class II fructose-bisphosphatase	-
LVJ74_RS14150 (LVJ74_14140)	3027856..3029280	+	1425	WP_003405805.1	class II fumarate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-85)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11049.76 Da
• Isoelectric Point: 4.9371

>T229122 WP_003898728.1 NZ_CP089779:3024450-3024761 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNTQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Nucleotide

• Length: 312 bp

>T229122 NZ_CP124400:c1711024-1710617 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11394.83 Da
• Isoelectric Point: 5.1236

>AT229122 WP_003405836.1 NZ_CP089779:3024130-3024450 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Nucleotide

• Length: 321 bp

>AT229122 NZ_CP124400:c1711271-1711005 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 5CCA
• PDB: 5UCT

Antitoxin

• AlphaFold DB: G0TGZ7