Detailed information of TA system TA229121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3015437..3016005
• Replicon: chromosome
• Accession: NZ_CP089779
• Organism: Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: LVJ74_RS14075
• Protein ID: WP_003405865.1
• Coordinates: 3015437..3015811 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: LVJ74_RS14080
• Protein ID: WP_003405863.1
• Coordinates: 3015808..3016005 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ74_RS14040 (LVJ74_14030)	3011787..3012557	+	771	Protein_2773	adenylate/guanylate cyclase domain-containing protein	-
LVJ74_RS14045 (LVJ74_14035)	3012509..3012718	-	210	WP_003911400.1	hypothetical protein	-
LVJ74_RS14050 (LVJ74_14040)	3012752..3013450	+	699	WP_031646953.1	hypothetical protein	-
LVJ74_RS14055 (LVJ74_14045)	3013465..3013788	-	324	WP_003405871.1	putative quinol monooxygenase	-
LVJ74_RS14060 (LVJ74_14050)	3014031..3014306	+	276	WP_003405867.1	hypothetical protein	-
LVJ74_RS14065 (LVJ74_14055)	3014233..3014418	-	186	WP_003901093.1	hypothetical protein	-
LVJ74_RS14070 (LVJ74_14060)	3014536..3015234	-	699	WP_003898733.1	hypothetical protein	-
LVJ74_RS14075 (LVJ74_14065)	3015437..3015811	-	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ74_RS14080 (LVJ74_14070)	3015808..3016005	-	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ74_RS14085 (LVJ74_14075)	3016093..3017166	-	1074	WP_003898732.1	redox-regulated ATPase YchF	-
LVJ74_RS14090 (LVJ74_14080)	3017229..3018212	+	984	WP_003898731.1	hypothetical protein	-
LVJ74_RS14095 (LVJ74_14085)	3018229..3019236	-	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LVJ74_RS14100 (LVJ74_14090)	3019326..3019964	+	639	WP_003898730.1	lipid droplet-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T229121 WP_003405865.1 NZ_CP089779:c3015811-3015437 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T229121 NZ_CP124400:c789361-789110 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT229121 WP_003405863.1 NZ_CP089779:c3016005-3015808 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT229121 NZ_CP124400:c789113-788859 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07