Detailed information of TA system TA229120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2870518..2871206
• Replicon: chromosome
• Accession: NZ_CP089779
• Organism: Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: LVJ74_RS13410
• Protein ID: WP_003406304.1
• Coordinates: 2870518..2870949 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: LVJ74_RS13415
• Protein ID: WP_003406302.1
• Coordinates: 2870946..2871206 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ74_RS13385 (LVJ74_13380)	2866240..2866509	+	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LVJ74_RS13390 (LVJ74_13385)	2866506..2866799	+	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
LVJ74_RS13395 (LVJ74_13390)	2866856..2867686	+	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
LVJ74_RS13400 (LVJ74_13395)	2867767..2868627	-	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
LVJ74_RS13405 (LVJ74_13400)	2868807..2870495	+	1689	WP_003910308.1	PE family protein	-
LVJ74_RS13410 (LVJ74_13405)	2870518..2870949	-	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ74_RS13415 (LVJ74_13410)	2870946..2871206	-	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
LVJ74_RS13420 (LVJ74_13415)	2871282..2872271	-	990	WP_003406301.1	malate dehydrogenase	-
LVJ74_RS13425 (LVJ74_13420)	2872442..2873542	+	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
LVJ74_RS13430 (LVJ74_13425)	2873619..2874800	-	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
LVJ74_RS13435 (LVJ74_13430)	2874805..2875629	-	825	WP_003900298.1	trehalose ABC transporter permease SugB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T229120 WP_003406304.1 NZ_CP089779:c2870949-2870518 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T229120 NZ_CP124400:c789113-788859 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT229120 WP_003406302.1 NZ_CP089779:c2871206-2870946 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT229120 NZ_CP124400:c789361-789110 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6