229117

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2682133..2682788 Replicon chromosome
Accession NZ_CP089779
Organism Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA6
Locus tag LVJ74_RS12590 Protein ID WP_003898861.1
Coordinates 2682387..2682788 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TIK2
Locus tag LVJ74_RS12585 Protein ID WP_003407272.1
Coordinates 2682133..2682390 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ74_RS12565 (LVJ74_12555) 2677320..2679287 - 1968 WP_003407285.1 primosomal protein N' -
LVJ74_RS12570 (LVJ74_12560) 2679368..2680105 - 738 WP_031647000.1 lysoplasmalogenase -
LVJ74_RS12575 (LVJ74_12565) 2680104..2681066 + 963 WP_003407279.1 alpha/beta hydrolase -
LVJ74_RS12580 (LVJ74_12570) 2681091..2682050 + 960 WP_003407276.1 alpha/beta hydrolase -
LVJ74_RS12585 (LVJ74_12575) 2682133..2682390 + 258 WP_003407272.1 CopG family transcriptional regulator Antitoxin
LVJ74_RS12590 (LVJ74_12580) 2682387..2682788 + 402 WP_003898861.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ74_RS12595 (LVJ74_12585) 2683043..2684773 + 1731 WP_010886114.1 PE family protein -
LVJ74_RS12600 (LVJ74_12590) 2684819..2685853 - 1035 WP_003407264.1 AraC family transcriptional regulator -
LVJ74_RS12605 (LVJ74_12595) 2685931..2687316 + 1386 WP_003407260.1 cytochrome P450 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-39)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14952.43 Da        Isoelectric Point: 11.4656

>T229117 WP_003898861.1 NZ_CP089779:2682387-2682788 [Mycobacterium tuberculosis]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQDLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Download         Length: 402 bp

>T229117 NZ_CP124400:c236012-234690 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCAGCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAACGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTACGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGATCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9443.58 Da        Isoelectric Point: 6.4631

>AT229117 WP_003407272.1 NZ_CP089779:2682133-2682390 [Mycobacterium tuberculosis]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Download         Length: 258 bp

>AT229117 NZ_CP124400:c236278-236012 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045ISB0

Antitoxin

Source ID Structure
AlphaFold DB G0TIK2

References