229116

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2569736..2570352 Replicon chromosome
Accession NZ_CP089779
Organism Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag LVJ74_RS12090 Protein ID WP_003407593.1
Coordinates 2569736..2570053 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag LVJ74_RS12095 Protein ID WP_003900349.1
Coordinates 2570050..2570352 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ74_RS12060 (LVJ74_12050) 2564745..2565773 - 1029 WP_003407612.1 glycosyltransferase family 2 protein -
LVJ74_RS12065 (LVJ74_12055) 2565818..2566216 - 399 WP_003900351.1 hypothetical protein -
LVJ74_RS12070 (LVJ74_12060) 2566277..2566489 + 213 WP_003898900.1 dodecin family protein -
LVJ74_RS12075 (LVJ74_12065) 2566610..2567320 + 711 WP_235639255.1 class I SAM-dependent methyltransferase -
LVJ74_RS12080 (LVJ74_12070) 2567393..2568682 - 1290 WP_003407599.1 serine hydrolase domain-containing protein -
LVJ74_RS12085 (LVJ74_12075) 2568735..2569739 - 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
LVJ74_RS12090 (LVJ74_12080) 2569736..2570053 - 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
LVJ74_RS12095 (LVJ74_12085) 2570050..2570352 - 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
LVJ74_RS12100 (LVJ74_12090) 2570366..2572618 - 2253 WP_003407587.1 methylmalonyl-CoA mutase -
LVJ74_RS12105 (LVJ74_12095) 2572619..2574466 - 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T229116 WP_003407593.1 NZ_CP089779:c2570053-2569736 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T229116 NZ_CP124389:31664-31912 [Escherichia coli]
ATGAGAAAAAATAAAGGCGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAAAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT229116 WP_003900349.1 NZ_CP089779:c2570352-2570050 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT229116 NZ_CP124389:31902-32129 [Escherichia coli]
ATGAAACCTGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
AlphaFold DB G0TJ73

References