Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2491238..2491851 Replicon chromosome
Accession NZ_CP089779
Organism Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)


Gene name vapC Uniprot ID P64880
Locus tag LVJ74_RS11745 Protein ID WP_003407786.1
Coordinates 2491238..2491642 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8UJ46
Locus tag LVJ74_RS11750 Protein ID WP_009935474.1
Coordinates 2491648..2491851 (-) Length 68 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ74_RS11735 (LVJ74_11720) 2487189..2489486 + 2298 WP_003407790.1 malto-oligosyltrehalose synthase -
LVJ74_RS11740 (LVJ74_11725) 2489479..2491221 + 1743 WP_003407788.1 malto-oligosyltrehalose trehalohydrolase -
LVJ74_RS11745 (LVJ74_11730) 2491238..2491642 - 405 WP_003407786.1 type II toxin-antitoxin system toxin ribonuclease VapC11 Toxin
LVJ74_RS11750 (LVJ74_11735) 2491648..2491851 - 204 WP_009935474.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ74_RS11755 (LVJ74_11740) 2491904..2493193 - 1290 WP_003407781.1 threonine ammonia-lyase -
LVJ74_RS11760 (LVJ74_11745) 2493228..2493674 - 447 WP_003407780.1 F420H(2)-dependent quinone reductase -
LVJ74_RS11765 (LVJ74_11750) 2493684..2494832 - 1149 Protein_2323 MMPL family transporter -
LVJ74_RS11770 (LVJ74_11755) 2495016..2495624 - 609 WP_003407773.1 TetR/AcrR family transcriptional regulator -
LVJ74_RS11775 (LVJ74_11760) 2495692..2496069 - 378 WP_003407771.1 fumarate reductase subunit FrdD -
LVJ74_RS11780 (LVJ74_11765) 2496066..2496446 - 381 WP_003407768.1 fumarate reductase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14650.76 Da        Isoelectric Point: 5.6587

>T229115 WP_003407786.1 NZ_CP089779:c2491642-2491238 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Download         Length: 405 bp

>T229115 NZ_CP124388:94781-95161 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 68 a.a.        Molecular weight: 7501.48 Da        Isoelectric Point: 4.3951

>AT229115 WP_009935474.1 NZ_CP089779:c2491851-2491648 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Download         Length: 204 bp

>AT229115 NZ_CP124388:94560-94781 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6A7V

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8UJ46

References