229114

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2308917..2309530 Replicon chromosome
Accession NZ_CP089779
Organism Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag LVJ74_RS10925 Protein ID WP_003408465.1
Coordinates 2309141..2309530 (+) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag LVJ74_RS10920 Protein ID WP_003408469.1
Coordinates 2308917..2309144 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ74_RS10900 (LVJ74_10885) 2304809..2305519 + 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -
LVJ74_RS10905 (LVJ74_10890) 2305509..2305928 + 420 WP_003408483.1 hypothetical protein -
LVJ74_RS10910 (LVJ74_10895) 2305971..2307218 - 1248 WP_003408476.1 serine hydrolase -
LVJ74_RS10915 (LVJ74_10900) 2307215..2308699 - 1485 WP_003408473.1 biotin carboxylase -
LVJ74_RS10920 (LVJ74_10905) 2308917..2309144 + 228 WP_003408469.1 antitoxin Antitoxin
LVJ74_RS10925 (LVJ74_10910) 2309141..2309530 + 390 WP_003408465.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ74_RS10930 (LVJ74_10915) 2309578..2309709 - 132 Protein_2158 IS3 family transposase -
LVJ74_RS10940 (LVJ74_10925) 2310140..2310919 - 780 WP_003408460.1 IclR family transcriptional regulator -
LVJ74_RS10945 (LVJ74_10930) 2310933..2311751 - 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
LVJ74_RS10950 (LVJ74_10935) 2311804..2312154 - 351 WP_003898986.1 cupin domain-containing protein -
LVJ74_RS10955 (LVJ74_10940) 2312154..2312984 - 831 WP_003916363.1 cyclase family protein -
LVJ74_RS10960 (LVJ74_10945) 2312987..2313901 - 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T229114 WP_003408465.1 NZ_CP089779:2309141-2309530 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T229114 NZ_CP124388:c89160-88855 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT229114 WP_003408469.1 NZ_CP089779:2308917-2309144 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT229114 NZ_CP124388:c89380-89162 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4FAN9

References