Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2066224..2066927 Replicon chromosome
Accession NZ_CP089779
Organism Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)


Gene name mazF Uniprot ID G0TLK7
Locus tag LVJ74_RS09830 Protein ID WP_003409778.1
Coordinates 2066598..2066927 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TLK8
Locus tag LVJ74_RS09825 Protein ID WP_003409780.1
Coordinates 2066224..2066601 (+) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ74_RS09785 (LVJ74_09765) 2061382..2061573 + 192 WP_003409876.1 hypothetical protein -
LVJ74_RS09790 (LVJ74_09770) 2061594..2062496 + 903 WP_003899097.1 hypothetical protein -
LVJ74_RS09795 (LVJ74_09775) 2062507..2062857 + 351 WP_003899096.1 hypothetical protein -
LVJ74_RS09800 (LVJ74_09780) 2062971..2063153 + 183 WP_003409870.1 hypothetical protein -
LVJ74_RS09805 (LVJ74_09785) 2063146..2063547 - 402 WP_003409869.1 hypothetical protein -
LVJ74_RS09810 (LVJ74_09790) 2063611..2064063 + 453 WP_003899095.1 lipoprotein -
LVJ74_RS09815 (LVJ74_09795) 2064218..2065582 - 1365 WP_003903691.1 HNH endonuclease signature motif containing protein -
LVJ74_RS09820 (LVJ74_09800) 2065637..2066227 + 591 WP_003409784.1 SEC-C metal-binding domain-containing protein -
LVJ74_RS09825 (LVJ74_09805) 2066224..2066601 + 378 WP_003409780.1 type II toxin-antitoxin system antitoxin MazE5 Antitoxin
LVJ74_RS09830 (LVJ74_09810) 2066598..2066927 + 330 WP_003409778.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LVJ74_RS09835 (LVJ74_09815) 2067137..2067907 - 771 WP_003409775.1 SDR family oxidoreductase -
LVJ74_RS09840 (LVJ74_09820) 2067904..2068965 - 1062 WP_003899092.1 3,4-dihydroxy-2-butanone-4-phosphate synthase -
LVJ74_RS09845 (LVJ74_09825) 2068962..2069477 - 516 WP_031658903.1 flavin reductase family protein -
LVJ74_RS09850 (LVJ74_09830) 2069474..2070544 - 1071 WP_003899091.1 epoxide hydrolase EphB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-103)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 11787.82 Da        Isoelectric Point: 8.5361

>T229112 WP_003409778.1 NZ_CP089779:2066598-2066927 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Download         Length: 330 bp

>T229112 NZ_CP124387:c4963905-4963798 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13560.07 Da        Isoelectric Point: 5.1519

>AT229112 WP_003409780.1 NZ_CP089779:2066224-2066601 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Download         Length: 378 bp

>AT229112 NZ_CP124387:4963953-4964019 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TLK7

Antitoxin

Source ID Structure
AlphaFold DB G0TLK8

References