229107

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2026679..2027265 Replicon chromosome
Accession NZ_CP089779
Organism Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag LVJ74_RS09555 Protein ID WP_003410010.1
Coordinates 2026921..2027265 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag LVJ74_RS09550 Protein ID WP_003410014.1
Coordinates 2026679..2026927 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ74_RS09530 (LVJ74_09510) 2022662..2023429 - 768 WP_003410019.1 hemerythrin domain-containing protein -
LVJ74_RS09535 (LVJ74_09515) 2023586..2023942 + 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
LVJ74_RS09540 (LVJ74_09520) 2023995..2024267 + 273 WP_003410017.1 DUF1490 family protein -
LVJ74_RS09545 (LVJ74_09525) 2024264..2026579 + 2316 WP_003899120.1 cation transporter ATPase CptG -
LVJ74_RS09550 (LVJ74_09530) 2026679..2026927 + 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
LVJ74_RS09555 (LVJ74_09535) 2026921..2027265 + 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
LVJ74_RS09560 (LVJ74_09540) 2027354..2027689 + 336 WP_003410009.1 dehydrogenase -
LVJ74_RS09565 (LVJ74_09545) 2027590..2027847 - 258 WP_003410006.1 hypothetical protein -
LVJ74_RS09570 (LVJ74_09550) 2027933..2028274 + 342 WP_003410003.1 DUF2384 domain-containing protein -
LVJ74_RS09575 (LVJ74_09555) 2028271..2028831 + 561 WP_003410001.1 RES family NAD+ phosphorylase -
LVJ74_RS09580 (LVJ74_09560) 2029351..2029890 - 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
LVJ74_RS09585 (LVJ74_09565) 2030116..2030544 - 429 WP_003409992.1 cellulose-binding protein -
LVJ74_RS09590 (LVJ74_09570) 2030960..2031559 - 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T229107 WP_003410010.1 NZ_CP089779:2026921-2027265 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T229107 NZ_CP124387:3557618-3557905 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT229107 WP_003410014.1 NZ_CP089779:2026679-2026927 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT229107 NZ_CP124387:3557892-3558194 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4FAW9

References