Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 1935105..1935741 Replicon chromosome
Accession NZ_CP089779
Organism Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag LVJ74_RS09160 Protein ID WP_003410654.1
Coordinates 1935105..1935515 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag LVJ74_RS09165 Protein ID WP_003410651.1
Coordinates 1935508..1935741 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ74_RS09140 (LVJ74_09120) 1930702..1931925 + 1224 WP_003901321.1 class I SAM-dependent methyltransferase -
LVJ74_RS09145 (LVJ74_09125) 1931871..1933397 - 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
LVJ74_RS09150 (LVJ74_09130) 1933394..1934020 - 627 WP_003410658.1 precorrin-8X methylmutase -
LVJ74_RS09155 (LVJ74_09135) 1934030..1935121 - 1092 WP_003900454.1 precorrin-3B synthase -
LVJ74_RS09160 (LVJ74_09140) 1935105..1935515 - 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
LVJ74_RS09165 (LVJ74_09145) 1935508..1935741 - 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
LVJ74_RS09170 (LVJ74_09150) 1935819..1939403 + 3585 WP_003410648.1 cobaltochelatase subunit CobN -
LVJ74_RS09175 (LVJ74_09155) 1939487..1939891 + 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
LVJ74_RS09180 (LVJ74_09160) 1939892..1940284 - 393 WP_229303073.1 metal ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T229104 WP_003410654.1 NZ_CP089779:c1935515-1935105 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T229104 NZ_CP124387:3275811-3275984 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT229104 WP_003410651.1 NZ_CP089779:c1935741-1935508 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT229104 NZ_CP124387:3275487-3275783 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References