Detailed information of TA system TA229093

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1192775..1193462
• Replicon: chromosome
• Accession: NZ_CP089779
• Organism: Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: LVJ74_RS05465
• Protein ID: WP_003414064.1
• Coordinates: 1192775..1193170 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: LVJ74_RS05470
• Protein ID: WP_003414061.1
• Coordinates: 1193196..1193462 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ74_RS05430 (LVJ74_05415)	1188672..1189409	-	738	WP_003414079.1	dienelactone hydrolase family protein	-
LVJ74_RS05435 (LVJ74_05420)	1189565..1190365	+	801	WP_003911953.1	thymidylate synthase	-
LVJ74_RS05440 (LVJ74_05425)	1190436..1190915	+	480	WP_003414073.1	dihydrofolate reductase	-
LVJ74_RS05445 (LVJ74_05430)	1190916..1190990	-	75	Protein_1075	hypothetical protein	-
LVJ74_RS05450 (LVJ74_05435)	1190989..1191408	+	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
LVJ74_RS05455 (LVJ74_05440)	1191405..1192499	+	1095	WP_003414068.1	restriction endonuclease subunit S	-
LVJ74_RS05460 (LVJ74_05445)	1192509..1192778	+	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
LVJ74_RS05465 (LVJ74_05450)	1192775..1193170	+	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ74_RS05470 (LVJ74_05455)	1193196..1193462	+	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ74_RS05475 (LVJ74_05460)	1193459..1193875	+	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
LVJ74_RS05480 (LVJ74_05465)	1193962..1195584	+	1623	WP_003906897.1	class I SAM-dependent DNA methyltransferase	-
LVJ74_RS05485 (LVJ74_05470)	1195581..1195856	+	276	WP_003414055.1	type I restriction endonuclease subunit S	-
LVJ74_RS05490 (LVJ74_05480)	1196100..1196852	+	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
LVJ74_RS05495 (LVJ74_05485)	1196921..1197823	+	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T229093 WP_003414064.1 NZ_CP089779:1192775-1193170 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T229093 NZ_CP124387:c791186-790932 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT229093 WP_003414061.1 NZ_CP089779:1193196-1193462 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT229093 NZ_CP124387:c791434-791183 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64