Detailed information of TA system TA229088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1080112..1080799
• Replicon: chromosome
• Accession: NZ_CP089779
• Organism: Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: LVJ74_RS04870
• Protein ID: WP_003414624.1
• Coordinates: 1080112..1080555 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: LVJ74_RS04875
• Protein ID: WP_003414620.1
• Coordinates: 1080542..1080799 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ74_RS04845 (LVJ74_04835)	1075960..1076226	-	267	WP_015456449.1	DUF2631 domain-containing protein	-
LVJ74_RS04850 (LVJ74_04840)	1076326..1076907	-	582	WP_003414644.1	fasciclin domain-containing protein	-
LVJ74_RS04855 (LVJ74_04845)	1077003..1078751	-	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
LVJ74_RS04860 (LVJ74_04850)	1079083..1079274	-	192	WP_031658948.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
LVJ74_RS04865 (LVJ74_04855)	1079370..1080032	-	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
LVJ74_RS04870 (LVJ74_04860)	1080112..1080555	-	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ74_RS04875 (LVJ74_04865)	1080542..1080799	-	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
LVJ74_RS04880 (LVJ74_04870)	1080926..1082167	+	1242	WP_031658947.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
LVJ74_RS04885 (LVJ74_04875)	1082175..1083389	+	1215	WP_003899518.1	zinc metalloprotease Rip	-
LVJ74_RS04890 (LVJ74_04880)	1083406..1084569	+	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
LVJ74_RS04895 (LVJ74_04885)	1084625..1085479	+	855	WP_003899516.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T229088 WP_003414624.1 NZ_CP089779:c1080555-1080112 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T229088 NZ_CP124386:c97775-97470 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT229088 WP_003414620.1 NZ_CP089779:c1080799-1080542 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT229088 NZ_CP124386:c97995-97777 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• AlphaFold DB: G0TFH0