Detailed information of TA system TA229085

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 487163..487692
• Replicon: chromosome
• Accession: NZ_CP089779
• Organism: Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: LVJ74_RS02290
• Protein ID: WP_003417760.1
• Coordinates: 487163..487420 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: LVJ74_RS02295
• Protein ID: WP_003417757.1
• Coordinates: 487417..487692 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ74_RS02260 (LVJ74_02245)	483591..483983	+	393	WP_003417776.1	serine protease inhibitor	-
LVJ74_RS02265 (LVJ74_02250)	483961..484329	+	369	WP_003417772.1	DUF742 domain-containing protein	-
LVJ74_RS02270 (LVJ74_02255)	484310..484891	+	582	WP_003417769.1	ATP/GTP-binding protein	-
LVJ74_RS02275 (LVJ74_02260)	484898..485449	+	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
LVJ74_RS02280 (LVJ74_02265)	485446..485814	-	369	WP_003417765.1	FHA domain-containing protein	-
LVJ74_RS02285 (LVJ74_02270)	485931..487121	-	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
LVJ74_RS02290 (LVJ74_02275)	487163..487420	-	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
LVJ74_RS02295 (LVJ74_02280)	487417..487692	-	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
LVJ74_RS02300 (LVJ74_02285)	487816..488661	+	846	WP_003417751.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
LVJ74_RS02305 (LVJ74_02290)	488658..488951	+	294	WP_031658983.1	DUF3017 domain-containing protein	-
LVJ74_RS02310 (LVJ74_02295)	488965..489354	-	390	WP_003417745.1	DUF732 domain-containing protein	-
LVJ74_RS02315 (LVJ74_02300)	489469..489729	+	261	WP_003417741.1	hypothetical protein	-
LVJ74_RS02320 (LVJ74_02305)	489756..490243	+	488	Protein_455	FAD-binding protein	-
LVJ74_RS02325 (LVJ74_02310)	490430..491119	+	690	WP_003917774.1	BBE domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T229085 WP_003417760.1 NZ_CP089779:c487420-487163 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T229085 NZ_CP124385:4359336-4359638 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT229085 WP_003417757.1 NZ_CP089779:c487692-487417 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT229085 NZ_CP124385:4359674-4360015 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• PDB: 3OEI

Antitoxin

• AlphaFold DB: G0TI94