229084

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-PHD
Location 459895..460562 Replicon chromosome
Accession NZ_CP089779
Organism Mycobacterium tuberculosis strain 01-R1134

Toxin (Protein)


Gene name vapC Uniprot ID O50411
Locus tag LVJ74_RS02160 Protein ID WP_003417916.1
Coordinates 460170..460562 (+) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8U8S7
Locus tag LVJ74_RS02155 Protein ID WP_003912214.1
Coordinates 459895..460170 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ74_RS02140 (LVJ74_02125) 455200..456072 + 873 WP_003417929.1 3-hydroxyacyl-thioester dehydratase HtdY -
LVJ74_RS02145 (LVJ74_02130) 456143..458152 - 2010 WP_031658985.1 PE family protein -
LVJ74_RS02150 (LVJ74_02135) 458342..459713 - 1372 Protein_421 ISNCY family transposase -
LVJ74_RS02155 (LVJ74_02140) 459895..460170 + 276 WP_003912214.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LVJ74_RS02160 (LVJ74_02145) 460170..460562 + 393 WP_003417916.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ74_RS02165 (LVJ74_02150) 461316..462368 + 1053 WP_003900678.1 polyprenyl synthetase family protein -
LVJ74_RS02170 (LVJ74_02155) 462368..463357 + 990 WP_003417912.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
LVJ74_RS02175 463354..463635 + 282 WP_003914149.1 1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein -
LVJ74_RS02180 463580..465190 + 1611 WP_003417910.1 1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 458342..459019 677


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14316.74 Da        Isoelectric Point: 10.7249

>T229084 WP_003417916.1 NZ_CP089779:460170-460562 [Mycobacterium tuberculosis]
MAAIYLDSSAIVKLAVREPESDALRRYLRTRHPRVSSALARAEVMRALLDKGESARKAGRRALAHLDLLRVDKRVLDLAG
GLLPFELRTLDAIHLATAQRLGVDLGRLCTYDDRMRDAAKTLGMAVIAPS

Download         Length: 393 bp

>T229084 NZ_CP124385:c4330379-4330161 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 9966.35 Da        Isoelectric Point: 4.5332

>AT229084 WP_003912214.1 NZ_CP089779:459895-460170 [Mycobacterium tuberculosis]
MTSVGVRALRQRASELLRRVEAGETIEITDRGRPVALLSPLPQGGPYEQLLASGEIERATLDVVDLPEPLDLDAGVELPS
VTLARLREHER

Download         Length: 276 bp

>AT229084 NZ_CP124385:c4330779-4330405 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BXS8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8U8S7

References