Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3192424..3193111 Replicon chromosome
Accession NZ_CP089778
Organism Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag LVJ73_RS15220 Protein ID WP_003414624.1
Coordinates 3192668..3193111 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag LVJ73_RS15215 Protein ID WP_003414620.1
Coordinates 3192424..3192681 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ73_RS15195 (LVJ73_15200) 3187744..3188598 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
LVJ73_RS15200 (LVJ73_15205) 3188654..3189817 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
LVJ73_RS15205 (LVJ73_15210) 3189834..3191048 - 1215 WP_003899518.1 zinc metalloprotease Rip -
LVJ73_RS15210 (LVJ73_15215) 3191056..3192297 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
LVJ73_RS15215 (LVJ73_15220) 3192424..3192681 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
LVJ73_RS15220 (LVJ73_15225) 3192668..3193111 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ73_RS15225 (LVJ73_15230) 3193191..3193853 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
LVJ73_RS15230 (LVJ73_15235) 3193949..3194140 + 192 WP_003414632.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
LVJ73_RS15235 (LVJ73_15240) 3194472..3196217 + 1746 WP_034163967.1 cytochrome c biogenesis protein DipZ -
LVJ73_RS15240 (LVJ73_15245) 3196313..3196894 + 582 WP_003414644.1 fasciclin domain-containing protein -
LVJ73_RS15245 (LVJ73_15250) 3196994..3197260 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T229077 WP_003414624.1 NZ_CP089778:3192668-3193111 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T229077 NZ_CP124385:3369377-3369550 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT229077 WP_003414620.1 NZ_CP089778:3192424-3192681 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT229077 NZ_CP124385:3369053-3369349 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References