Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3145908..3146512 Replicon chromosome
Accession NZ_CP089778
Organism Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag LVJ73_RS14990 Protein ID WP_003414492.1
Coordinates 3145908..3146300 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag LVJ73_RS14995 Protein ID WP_003414495.1
Coordinates 3146297..3146512 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ73_RS14960 (LVJ73_14970) 3141058..3141846 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
LVJ73_RS14965 (LVJ73_14975) 3142180..3142725 - 546 WP_003913758.1 DUF1802 family protein -
LVJ73_RS14970 (LVJ73_14980) 3142997..3143881 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
LVJ73_RS14975 (LVJ73_14985) 3143884..3144771 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
LVJ73_RS14980 (LVJ73_14990) 3145076..3145621 - 546 WP_003899500.1 DUF1802 family protein -
LVJ73_RS14985 (LVJ73_14995) 3145618..3145887 - 270 WP_003414489.1 DUF2277 family protein -
LVJ73_RS14990 (LVJ73_15000) 3145908..3146300 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ73_RS14995 (LVJ73_15005) 3146297..3146512 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LVJ73_RS15000 (LVJ73_15010) 3146559..3147308 + 750 WP_003414497.1 enoyl-CoA hydratase -
LVJ73_RS15005 (LVJ73_15015) 3147387..3148469 - 1083 WP_003414499.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
LVJ73_RS15010 (LVJ73_15020) 3148462..3149772 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
LVJ73_RS15015 (LVJ73_15025) 3149775..3150602 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
LVJ73_RS15020 (LVJ73_15030) 3150599..3151510 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T229075 WP_003414492.1 NZ_CP089778:c3146300-3145908 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T229075 NZ_CP124385:c2656825-2656718 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT229075 WP_003414495.1 NZ_CP089778:c3146512-3146297 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT229075 NZ_CP124385:2656874-2656939 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References