Detailed information of TA system TA229074

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii-AbiEi
• Location: 3142997..3144771
• Replicon: chromosome
• Accession: NZ_CP089778
• Organism: Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: P71626
• Locus tag: LVJ73_RS14970
• Protein ID: WP_003414409.1
• Coordinates: 3142997..3143881 (-)
• Length: 295 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: P71625
• Locus tag: LVJ73_RS14975
• Protein ID: WP_003414414.1
• Coordinates: 3143884..3144771 (-)
• Length: 296 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ73_RS14950 (LVJ73_14960)	3138252..3138626	-	375	WP_003414296.1	type III-A CRISPR-associated protein Csm2	-
LVJ73_RS14955 (LVJ73_14965)	3138623..3141061	-	2439	WP_003900580.1	type III-A CRISPR-associated protein Cas10/Csm1	-
LVJ73_RS14960 (LVJ73_14970)	3141058..3141846	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
LVJ73_RS14965 (LVJ73_14975)	3142180..3142725	-	546	WP_003913758.1	DUF1802 family protein	-
LVJ73_RS14970 (LVJ73_14980)	3142997..3143881	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LVJ73_RS14975 (LVJ73_14985)	3143884..3144771	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
LVJ73_RS14980 (LVJ73_14990)	3145076..3145621	-	546	WP_003899500.1	DUF1802 family protein	-
LVJ73_RS14985 (LVJ73_14995)	3145618..3145887	-	270	WP_003414489.1	DUF2277 family protein	-
LVJ73_RS14990 (LVJ73_15000)	3145908..3146300	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	-
LVJ73_RS14995 (LVJ73_15005)	3146297..3146512	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LVJ73_RS15000 (LVJ73_15010)	3146559..3147308	+	750	WP_003414497.1	enoyl-CoA hydratase	-
LVJ73_RS15005 (LVJ73_15015)	3147387..3148469	-	1083	WP_003414499.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (36-272)

Antitoxin

• AbiEi antitoxin C-terminal domain (84-237)

Sequences

Toxin

Protein

• Length: 295 a.a.
• Molecular weight: 33056.72 Da
• Isoelectric Point: 7.1520

>T229074 WP_003414409.1 NZ_CP089778:c3143881-3142997 [Mycobacterium tuberculosis]
VAGLTRALVARHALGRAEAYDAALLDVAQDHLLYLLSQTVQFGDNRLVFKGGTSLRKCRLGNVGRFSTDLDFSAPDDEVV
LEVCELIDGARVGGFEFGVQSTRGDGRHWQLRVRHTELGEPRIVASVEFARRPLALPSELLAFIQLPIHKAYGFGLPTLP
VVAEAEACAEKLARYRRVALARDLYDLNHFASRTIDEPLVRRLWVLKVWGDVVDDRRGTRPLRVEDVLAARSEHDFQPDS
IGVLTRPVAMAAWEARVRKRFAFLTDLDADEQRWAACDERHRREVENALAVLRS

Nucleotide

• Length: 885 bp

>T229074 NZ_CP124385:c2656342-2656235 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 296 a.a.
• Molecular weight: 32315.19 Da
• Isoelectric Point: 9.4723

>AT229074 WP_003414414.1 NZ_CP089778:c3144771-3143884 [Mycobacterium tuberculosis]
VVSPAGADRRIPTWASRVVSGLARDRPVVVTKEDLTQRLTEAGCGRDPDSAIRELRRIGWLVQLPVKGTWAFIPPGEAAI
SDPYLPLRSWLARDQNAGFMLAGASAAWHLGYLDRQPDGRIPIWLPPAKRLPDGLASYVSVVRIPWNAADTALLAPRPAL
LVRRRLDLVAWATGLPALGPEALLVQIATRPASFGPWADLVPHLDDLVADCSDERLERLLSGRPTSAWQRASYLLDSGGE
PARGQALLAKRHTEVMPVTRFTTAHSRDRGESVWAPEYQLVDELVVPLLRVIGKA

Nucleotide

• Length: 888 bp

>AT229074 NZ_CP124385:2656399-2656456 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Toxin

• PDB: 6Y56

Antitoxin

• AlphaFold DB: A0A829CBU9