Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2879776..2880407 Replicon chromosome
Accession NZ_CP089778
Organism Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag LVJ73_RS13520 Protein ID WP_003413174.1
Coordinates 2880030..2880407 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag LVJ73_RS13515 Protein ID WP_003413167.1
Coordinates 2879776..2880033 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ73_RS13485 (LVJ73_13490) 2875690..2876004 + 315 WP_009937839.1 hypothetical protein -
LVJ73_RS13490 (LVJ73_13495) 2876300..2877511 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
LVJ73_RS13495 (LVJ73_13500) 2877638..2878297 + 660 WP_003900846.1 LppA family lipoprotein -
LVJ73_RS13500 (LVJ73_13505) 2878294..2878956 + 663 WP_003900848.1 LppA family lipoprotein -
LVJ73_RS13505 (LVJ73_13510) 2878953..2879231 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ73_RS13510 (LVJ73_13515) 2879324..2879737 + 414 WP_003413164.1 PIN domain nuclease -
LVJ73_RS13515 (LVJ73_13520) 2879776..2880033 + 258 WP_003413167.1 CopG family transcriptional regulator Antitoxin
LVJ73_RS13520 (LVJ73_13525) 2880030..2880407 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ73_RS13525 (LVJ73_13530) 2880423..2880797 - 375 WP_003413177.1 hypothetical protein -
LVJ73_RS13530 (LVJ73_13535) 2880897..2881292 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
LVJ73_RS13535 (LVJ73_13540) 2881289..2881534 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
LVJ73_RS13540 (LVJ73_13545) 2881945..2882364 - 420 WP_003413190.1 A24 family peptidase -
LVJ73_RS13545 (LVJ73_13550) 2882376..2883185 - 810 WP_003413193.1 shikimate dehydrogenase -
LVJ73_RS13550 (LVJ73_13555) 2883182..2884438 - 1257 WP_014585306.1 endolytic transglycosylase MltG -
LVJ73_RS13555 (LVJ73_13560) 2884431..2884943 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T229068 WP_003413174.1 NZ_CP089778:2880030-2880407 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T229068 NZ_CP124385:c919186-918935 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT229068 WP_003413167.1 NZ_CP089778:2879776-2880033 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT229068 NZ_CP124385:c918938-918684 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References