Detailed information of TA system TA229066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2865438..2866078
• Replicon: chromosome
• Accession: NZ_CP089778
• Organism: Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: LVJ73_RS13425
• Protein ID: WP_003412970.1
• Coordinates: 2865438..2865857 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: LVJ73_RS13430
• Protein ID: WP_003412975.1
• Coordinates: 2865854..2866078 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ73_RS13395 (LVJ73_13400)	2861023..2861745	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
LVJ73_RS13400 (LVJ73_13405)	2862262..2862489	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
LVJ73_RS13405 (LVJ73_13410)	2862486..2862887	+	402	WP_003412963.1	PIN domain-containing protein	-
LVJ73_RS13410 (LVJ73_13415)	2862922..2863842	-	921	WP_003412965.1	restriction endonuclease	-
LVJ73_RS13415 (LVJ73_13420)	2864183..2864428	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LVJ73_RS13420 (LVJ73_13425)	2864487..2865437	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
LVJ73_RS13425 (LVJ73_13430)	2865438..2865857	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ73_RS13430 (LVJ73_13435)	2865854..2866078	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
LVJ73_RS13435 (LVJ73_13440)	2866109..2868951	-	2843	Protein_2652	amino acid decarboxylase	-
LVJ73_RS13440 (LVJ73_13445)	2869023..2869424	-	402	WP_003412981.1	hypothetical protein	-
LVJ73_RS13445 (LVJ73_13450)	2869424..2869894	-	471	WP_003899364.1	transcription antitermination factor NusB	-
LVJ73_RS13450 (LVJ73_13455)	2869897..2870460	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T229066 WP_003412970.1 NZ_CP089778:c2865857-2865438 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T229066 NZ_CP124385:866682-867056 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT229066 WP_003412975.1 NZ_CP089778:c2866078-2865854 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT229066 NZ_CP124385:866225-866593 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09