Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2333306..2333942 Replicon chromosome
Accession NZ_CP089778
Organism Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag LVJ73_RS10895 Protein ID WP_003410654.1
Coordinates 2333532..2333942 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag LVJ73_RS10890 Protein ID WP_003410651.1
Coordinates 2333306..2333539 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ73_RS10875 (LVJ73_10890) 2328763..2329155 + 393 WP_229303073.1 metal ABC transporter permease -
LVJ73_RS10880 (LVJ73_10895) 2329156..2329560 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
LVJ73_RS10885 (LVJ73_10900) 2329644..2333228 - 3585 WP_003410648.1 cobaltochelatase subunit CobN -
LVJ73_RS10890 (LVJ73_10905) 2333306..2333539 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
LVJ73_RS10895 (LVJ73_10910) 2333532..2333942 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
LVJ73_RS10900 (LVJ73_10915) 2333926..2335017 + 1092 WP_003900454.1 precorrin-3B synthase -
LVJ73_RS10905 (LVJ73_10920) 2335027..2335653 + 627 WP_003410658.1 precorrin-8X methylmutase -
LVJ73_RS10910 (LVJ73_10925) 2335650..2337176 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
LVJ73_RS10915 (LVJ73_10930) 2337122..2338345 - 1224 WP_003899152.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T229062 WP_003410654.1 NZ_CP089778:2333532-2333942 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T229062 NZ_CP124376:c63517-63137 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGATATCACCGATCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCAGAACTGGCAGACCTTACCGTAGGGGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT229062 WP_003410651.1 NZ_CP089778:2333306-2333539 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT229062 NZ_CP124376:c63738-63517 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References