Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2202118..2202821 Replicon chromosome
Accession NZ_CP089778
Organism Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)


Gene name mazF Uniprot ID G0TLK7
Locus tag LVJ73_RS10225 Protein ID WP_003409778.1
Coordinates 2202118..2202447 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TLK8
Locus tag LVJ73_RS10230 Protein ID WP_003409780.1
Coordinates 2202444..2202821 (-) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ73_RS10205 (LVJ73_10220) 2198501..2199571 + 1071 WP_003899091.1 epoxide hydrolase EphB -
LVJ73_RS10210 (LVJ73_10225) 2199568..2200083 + 516 WP_003409718.1 flavin reductase family protein -
LVJ73_RS10215 (LVJ73_10230) 2200080..2201141 + 1062 WP_003899092.1 3,4-dihydroxy-2-butanone-4-phosphate synthase -
LVJ73_RS10220 (LVJ73_10235) 2201138..2201908 + 771 WP_003409775.1 SDR family oxidoreductase -
LVJ73_RS10225 (LVJ73_10240) 2202118..2202447 - 330 WP_003409778.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LVJ73_RS10230 (LVJ73_10245) 2202444..2202821 - 378 WP_003409780.1 type II toxin-antitoxin system antitoxin MazE5 Antitoxin
LVJ73_RS10235 (LVJ73_10250) 2202818..2203408 - 591 WP_003409784.1 SEC-C metal-binding domain-containing protein -
LVJ73_RS10240 (LVJ73_10255) 2203463..2204827 + 1365 WP_070889705.1 HNH endonuclease signature motif containing protein -
LVJ73_RS10245 (LVJ73_10260) 2204982..2205434 - 453 WP_003899095.1 lipoprotein -
LVJ73_RS10250 (LVJ73_10265) 2205498..2205899 + 402 WP_003409869.1 hypothetical protein -
LVJ73_RS10255 (LVJ73_10270) 2205892..2206074 - 183 WP_003409870.1 hypothetical protein -
LVJ73_RS10260 (LVJ73_10275) 2206188..2206538 - 351 WP_003899096.1 hypothetical protein -
LVJ73_RS10265 (LVJ73_10280) 2206549..2207451 - 903 WP_003899097.1 hypothetical protein -
LVJ73_RS10270 (LVJ73_10285) 2207472..2207663 - 192 WP_003409876.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-103)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 11787.82 Da        Isoelectric Point: 8.5361

>T229054 WP_003409778.1 NZ_CP089778:c2202447-2202118 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Download         Length: 330 bp

>T229054 NZ_CP124374:c4658170-4658063 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13560.07 Da        Isoelectric Point: 5.1519

>AT229054 WP_003409780.1 NZ_CP089778:c2202821-2202444 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Download         Length: 378 bp

>AT229054 NZ_CP124374:4658227-4658284 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TLK7

Antitoxin

Source ID Structure
AlphaFold DB G0TLK8

References