Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1765491..1766104 Replicon chromosome
Accession NZ_CP089778
Organism Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)


Gene name vapC Uniprot ID P64880
Locus tag LVJ73_RS08285 Protein ID WP_003407786.1
Coordinates 1765700..1766104 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8UJ46
Locus tag LVJ73_RS08280 Protein ID WP_009935474.1
Coordinates 1765491..1765694 (+) Length 68 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ73_RS08250 (LVJ73_08260) 1760896..1761276 + 381 WP_003407768.1 fumarate reductase subunit C -
LVJ73_RS08255 (LVJ73_08265) 1761273..1761650 + 378 WP_003407771.1 fumarate reductase subunit FrdD -
LVJ73_RS08260 (LVJ73_08270) 1761718..1762326 + 609 WP_003407773.1 TetR/AcrR family transcriptional regulator -
LVJ73_RS08265 (LVJ73_08275) 1762510..1763658 + 1149 Protein_1630 MMPL family transporter -
LVJ73_RS08270 (LVJ73_08280) 1763668..1764114 + 447 WP_053869442.1 F420H(2)-dependent quinone reductase -
LVJ73_RS08275 (LVJ73_08285) 1764149..1765438 + 1290 WP_003407781.1 threonine ammonia-lyase -
LVJ73_RS08280 (LVJ73_08290) 1765491..1765694 + 204 WP_009935474.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ73_RS08285 (LVJ73_08295) 1765700..1766104 + 405 WP_003407786.1 type II toxin-antitoxin system toxin ribonuclease VapC11 Toxin
LVJ73_RS08290 (LVJ73_08300) 1766121..1767863 - 1743 WP_003407788.1 malto-oligosyltrehalose trehalohydrolase -
LVJ73_RS08295 (LVJ73_08305) 1767856..1770153 - 2298 WP_003407790.1 malto-oligosyltrehalose synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14650.76 Da        Isoelectric Point: 5.6587

>T229051 WP_003407786.1 NZ_CP089778:1765700-1766104 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Download         Length: 405 bp

>T229051 NZ_CP124374:c4202913-4202599 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 68 a.a.        Molecular weight: 7501.48 Da        Isoelectric Point: 4.3951

>AT229051 WP_009935474.1 NZ_CP089778:1765491-1765694 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Download         Length: 204 bp

>AT229051 NZ_CP124374:c4202602-4202186 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6A7V

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8UJ46

References