Detailed information of TA system TA229047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1388723..1389282
• Replicon: chromosome
• Accession: NZ_CP089778
• Organism: Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0THS1
• Locus tag: LVJ73_RS06635
• Protein ID: WP_003898789.1
• Coordinates: 1388723..1389016 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0THS2
• Locus tag: LVJ73_RS06640
• Protein ID: WP_003406322.1
• Coordinates: 1389013..1389282 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ73_RS06610 (LVJ73_06605)	1384316..1384576	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	-
LVJ73_RS06615 (LVJ73_06610)	1384573..1385004	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	-
LVJ73_RS06620 (LVJ73_06615)	1385027..1386715	-	1689	WP_003910308.1	PE family protein	-
LVJ73_RS06625 (LVJ73_06620)	1386895..1387755	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
LVJ73_RS06630 (LVJ73_06625)	1387836..1388666	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
LVJ73_RS06635 (LVJ73_06630)	1388723..1389016	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
LVJ73_RS06640 (LVJ73_06635)	1389013..1389282	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LVJ73_RS06645 (LVJ73_06640)	1389395..1393090	-	3696	WP_003898790.1	multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit	-
LVJ73_RS06650 (LVJ73_06645)	1393232..1394020	-	789	WP_003406325.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11031.57 Da
• Isoelectric Point: 9.6275

>T229047 WP_003898789.1 NZ_CP089778:c1389016-1388723 [Mycobacterium tuberculosis]
VSDDHPYHVAITATAARDLQRLPEKIAAACVEFVFGPLLNNPHRLGKPLRNDLEGLHSARRGDYRVVYAIDDGHHRVEII
HIARRSASYRMNPCRPR

Nucleotide

• Length: 294 bp

>T229047 NZ_CP124374:c2990570-2990316 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9766.97 Da
• Isoelectric Point: 4.8615

>AT229047 WP_003406322.1 NZ_CP089778:c1389282-1389013 [Mycobacterium tuberculosis]
MAVVPLGEVRNRLSEYVAEVELTHERITITRHGHPAAVLISADDLASIEETLEVLRTPGASEAIREGLADVAAGRFVSND
EIRNRYTAR

Nucleotide

• Length: 270 bp

>AT229047 NZ_CP124374:c2990818-2990567 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: G0THS1

Antitoxin

• AlphaFold DB: G0THS2