Detailed information of TA system TA229046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1384316..1385004
• Replicon: chromosome
• Accession: NZ_CP089778
• Organism: Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: LVJ73_RS06615
• Protein ID: WP_003406304.1
• Coordinates: 1384573..1385004 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: LVJ73_RS06610
• Protein ID: WP_003406302.1
• Coordinates: 1384316..1384576 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ73_RS06590 (LVJ73_06585)	1379893..1380717	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
LVJ73_RS06595 (LVJ73_06590)	1380722..1381903	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
LVJ73_RS06600 (LVJ73_06595)	1381980..1383080	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
LVJ73_RS06605 (LVJ73_06600)	1383251..1384240	+	990	WP_053869428.1	malate dehydrogenase	-
LVJ73_RS06610 (LVJ73_06605)	1384316..1384576	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
LVJ73_RS06615 (LVJ73_06610)	1384573..1385004	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ73_RS06620 (LVJ73_06615)	1385027..1386715	-	1689	WP_003910308.1	PE family protein	-
LVJ73_RS06625 (LVJ73_06620)	1386895..1387755	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
LVJ73_RS06630 (LVJ73_06625)	1387836..1388666	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
LVJ73_RS06635 (LVJ73_06630)	1388723..1389016	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
LVJ73_RS06640 (LVJ73_06635)	1389013..1389282	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T229046 WP_003406304.1 NZ_CP089778:1384573-1385004 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T229046 NZ_CP124374:2979409-2979783 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT229046 WP_003406302.1 NZ_CP089778:1384316-1384576 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT229046 NZ_CP124374:2978952-2979320 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6