Detailed information of TA system TA229045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239446..1240014
• Replicon: chromosome
• Accession: NZ_CP089778
• Organism: Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: LVJ73_RS05940
• Protein ID: WP_003405865.1
• Coordinates: 1239640..1240014 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: LVJ73_RS05935
• Protein ID: WP_003405863.1
• Coordinates: 1239446..1239643 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ73_RS05915 (LVJ73_05915)	1235487..1236125	-	639	WP_003898730.1	lipid droplet-associated protein	-
LVJ73_RS05920 (LVJ73_05920)	1236215..1237222	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LVJ73_RS05925 (LVJ73_05925)	1237239..1238222	-	984	WP_003898731.1	hypothetical protein	-
LVJ73_RS05930 (LVJ73_05930)	1238285..1239358	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
LVJ73_RS05935 (LVJ73_05935)	1239446..1239643	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ73_RS05940 (LVJ73_05940)	1239640..1240014	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ73_RS05945 (LVJ73_05945)	1240217..1240915	+	699	WP_003898733.1	hypothetical protein	-
LVJ73_RS05950 (LVJ73_05950)	1241033..1241218	+	186	WP_003901093.1	hypothetical protein	-
LVJ73_RS05955 (LVJ73_05955)	1241145..1241420	-	276	WP_003405867.1	hypothetical protein	-
LVJ73_RS05960 (LVJ73_05960)	1241663..1241986	+	324	WP_003405871.1	putative quinol monooxygenase	-
LVJ73_RS05965 (LVJ73_05965)	1242001..1242699	-	699	WP_031646953.1	hypothetical protein	-
LVJ73_RS05970 (LVJ73_05970)	1242733..1242942	+	210	WP_003911400.1	hypothetical protein	-
LVJ73_RS05975 (LVJ73_05975)	1242894..1243664	-	771	Protein_1177	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T229045 WP_003405865.1 NZ_CP089778:1239640-1240014 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T229045 NZ_CP124374:c2717018-2716916 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT229045 WP_003405863.1 NZ_CP089778:1239446-1239643 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT229045 NZ_CP124374:2716878-2717146 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07