Detailed information of TA system TA229042

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1024306..1025279
• Replicon: chromosome
• Accession: NZ_CP089778
• Organism: Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: O05911
• Locus tag: LVJ73_RS04870
• Protein ID: WP_003404756.1
• Coordinates: 1024779..1025279 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: LVJ73_RS04865
• Protein ID: WP_053869509.1
• Coordinates: 1024306..1024782 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ73_RS04845 (LVJ73_04840)	1020153..1021424	-	1272	WP_070889687.1	PPE family protein	-
LVJ73_RS04850 (LVJ73_04845)	1021439..1021738	-	300	WP_003404748.1	PE family protein	-
LVJ73_RS04855 (LVJ73_04850)	1022182..1023963	+	1782	WP_003404749.1	BCCT family transporter	-
LVJ73_RS04860 (LVJ73_04855)	1023963..1024217	+	255	WP_003898646.1	hypothetical protein	-
LVJ73_RS04865 (LVJ73_04860)	1024306..1024782	+	477	WP_053869509.1	DUF1778 domain-containing protein	Antitoxin
LVJ73_RS04870 (LVJ73_04865)	1024779..1025279	+	501	WP_003404756.1	GNAT family N-acetyltransferase	Toxin
LVJ73_RS04880 (LVJ73_04875)	1025592..1026911	-	1320	WP_003404757.1	IS256-like element IS1554 family transposase	-
LVJ73_RS04885 (LVJ73_04880)	1027199..1027780	+	582	WP_003404760.1	IS607-like element IS1535 family transposase	-
LVJ73_RS04890 (LVJ73_04885)	1027780..1029432	+	1653	WP_003404761.1	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1023960..1069871	45911

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (65-156)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18048.55 Da
• Isoelectric Point: 7.8659

>T229042 WP_003404756.1 NZ_CP089778:1024779-1025279 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Nucleotide

• Length: 501 bp

>T229042 NZ_CP124374:1237933-1238235 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 17357.95 Da
• Isoelectric Point: 11.2467

>AT229042 WP_053869509.1 NZ_CP089778:1024306-1024782 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGSSGLPALNCTPIRHAIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Nucleotide

• Length: 477 bp

>AT229042 NZ_CP124374:1238271-1238612 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: A0A7U4BSZ2