Detailed information of TA system TA229041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1014776..1015395
• Replicon: chromosome
• Accession: NZ_CP089778
• Organism: Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: LVJ73_RS04815
• Protein ID: WP_003404726.1
• Coordinates: 1014961..1015395 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: LVJ73_RS04810
• Protein ID: WP_003898641.1
• Coordinates: 1014776..1014955 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ73_RS04795 (LVJ73_04790)	1010249..1011829	+	1581	WP_003909313.1	serine hydrolase	-
LVJ73_RS04800 (LVJ73_04795)	1011826..1014219	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
LVJ73_RS04805 (LVJ73_04800)	1014492..1014650	+	159	WP_003404720.1	hypothetical protein	-
LVJ73_RS04810 (LVJ73_04805)	1014776..1014955	+	180	WP_003898641.1	antitoxin	Antitoxin
LVJ73_RS04815 (LVJ73_04810)	1014961..1015395	+	435	WP_003404726.1	SRPBCC family protein	Toxin
LVJ73_RS04820 (LVJ73_04815)	1015493..1016266	+	774	WP_003404735.1	VOC family protein	-
LVJ73_RS04825 (LVJ73_04820)	1016331..1016780	+	450	WP_003404738.1	hypothetical protein	-
LVJ73_RS04830 (LVJ73_04825)	1016915..1017145	-	231	WP_003898642.1	hypothetical protein	-
LVJ73_RS04835 (LVJ73_04830)	1017312..1018820	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
LVJ73_RS04840 (LVJ73_04835)	1018822..1020060	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T229041 WP_003404726.1 NZ_CP089778:1014961-1015395 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T229041 NZ_CP124374:c1208979-1208761 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT229041 WP_003898641.1 NZ_CP089778:1014776-1014955 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT229041 NZ_CP124374:c1209379-1209005 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5