Detailed information of TA system TA022904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1439785..1440329
• Replicon: chromosome
• Accession: NC_010803
• Organism: Chlorobium limicola DSM 245

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CLIM_RS06715
• Protein ID: WP_041465707.1
• Coordinates: 1439785..1440120 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B3ECY3
• Locus tag: CLIM_RS06720
• Protein ID: WP_012466285.1
• Coordinates: 1440117..1440329 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLIM_RS06660	1435495..1435704	+	210	WP_081429881.1	type II toxin-antitoxin system PemK/MazF family toxin	-
CLIM_RS12725	1435745..1436098	+	354	WP_150081611.1	GNAT family N-acetyltransferase	-
CLIM_RS06670	1436612..1436920	+	309	WP_012466275.1	type II toxin-antitoxin system MqsR family toxin	-
CLIM_RS06675	1436958..1437368	+	411	WP_012466276.1	type II toxin-antitoxin system MqsA family antitoxin	-
CLIM_RS06680	1437546..1437806	-	261	WP_012466277.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CLIM_RS06685	1437790..1438014	-	225	WP_041465944.1	ribbon-helix-helix protein, CopG family	-
CLIM_RS06695	1438462..1438731	+	270	WP_012466280.1	DUF4160 domain-containing protein	-
CLIM_RS06700	1438735..1438977	+	243	WP_012466281.1	DUF2442 domain-containing protein	-
CLIM_RS06705	1439081..1439350	+	270	WP_041465706.1	hypothetical protein	-
CLIM_RS06710	1439347..1439646	+	300	WP_012466283.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CLIM_RS06715	1439785..1440120	-	336	WP_041465707.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CLIM_RS06720	1440117..1440329	-	213	WP_012466285.1	antitoxin MazE family protein	Antitoxin
CLIM_RS06725	1440927..1441169	+	243	WP_012466286.1	ribbon-helix-helix domain-containing protein	-
CLIM_RS06730	1441154..1441492	+	339	WP_012466287.1	type II toxin-antitoxin system PemK/MazF family toxin	-
CLIM_RS06735	1441537..1441785	-	249	WP_012466288.1	hypothetical protein	-
CLIM_RS12730	1441999..1444902	-	2904	WP_012466289.1	DEAD/DEAH box helicase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1423740..1471517	47777

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (6-68)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12121.13 Da
• Isoelectric Point: 9.6920

>T22904 WP_041465707.1 NC_010803:c1440120-1439785 [Chlorobium limicola DSM 245]
MKRGSVVTIALQGDYGKPRPALVVQSDYFSGHPSMTVLPITSELRQTPLFRIDVEPESGNGLRKRSQIMVDKIQTVPAEK
IVKAIGILDEATMMEVNRALALWLGFAGNPG

Nucleotide

• Length: 336 bp

>T22904 NC_010803:c1440120-1439785 [Chlorobium limicola DSM 245]
ATGAAGCGCGGGTCTGTAGTGACGATCGCATTGCAGGGTGATTATGGTAAACCTCGGCCCGCATTGGTTGTGCAGTCTGA
TTATTTCTCAGGACATCCATCGATGACTGTTTTACCGATTACCAGTGAGTTGCGTCAGACGCCGTTATTCAGAATCGATG
TCGAGCCTGAATCCGGTAATGGATTGCGTAAGCGTTCCCAGATCATGGTCGATAAAATACAGACTGTTCCGGCAGAAAAA
ATCGTCAAAGCCATCGGTATTCTTGACGAAGCAACCATGATGGAAGTCAACAGGGCGCTTGCGCTTTGGTTAGGATTTGC
CGGTAATCCAGGGTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8417.58 Da
• Isoelectric Point: 9.1636

>AT22904 WP_012466285.1 NC_010803:c1440329-1440117 [Chlorobium limicola DSM 245]
MKVVTERVKRYRDRLRERGLRPVQIWVADTRRPGFEQECRRQSALLKSDALEKEVLEFLEQASDREGWEA

Nucleotide

• Length: 213 bp

>AT22904 NC_010803:c1440329-1440117 [Chlorobium limicola DSM 245]
ATGAAAGTTGTTACAGAAAGGGTAAAGCGGTACAGGGACAGGCTTCGGGAGCGCGGATTGAGACCGGTGCAGATATGGGT
GGCGGATACGCGGCGTCCCGGATTCGAACAGGAGTGCCGCCGTCAATCGGCGCTGCTCAAGTCGGATGCTCTTGAAAAAG
AGGTTCTTGAATTTCTTGAGCAGGCCTCAGATCGGGAAGGTTGGGAAGCATGA

Structures

Antitoxin

• AlphaFold DB: B3ECY3