Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 756347..756887 | Replicon | chromosome |
| Accession | NZ_CP089778 | ||
| Organism | Mycobacterium tuberculosis strain I0003758-5 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | P9WII0 |
| Locus tag | LVJ73_RS03490 | Protein ID | WP_003403376.1 |
| Coordinates | 756347..756655 (-) | Length | 103 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | G0TQE0 |
| Locus tag | LVJ73_RS03495 | Protein ID | WP_003403381.1 |
| Coordinates | 756642..756887 (-) | Length | 82 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LVJ73_RS03465 (LVJ73_03460) | 751662..753167 | + | 1506 | WP_003403360.1 | carotenoid cleavage oxygenase | - |
| LVJ73_RS03470 (LVJ73_03465) | 753248..754258 | + | 1011 | WP_003911248.1 | ABC transporter ATP-binding protein | - |
| LVJ73_RS03475 (LVJ73_03470) | 754646..755029 | - | 384 | WP_003403365.1 | ribonuclease VapC6 | - |
| LVJ73_RS03480 (LVJ73_03475) | 755124..755279 | - | 156 | WP_003403368.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| LVJ73_RS03485 (LVJ73_03480) | 755355..756071 | - | 717 | WP_003403371.1 | CPBP family intramembrane metalloprotease | - |
| LVJ73_RS03490 (LVJ73_03485) | 756347..756655 | - | 309 | WP_003403376.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| LVJ73_RS03495 (LVJ73_03490) | 756642..756887 | - | 246 | WP_003403381.1 | ribbon-helix-helix protein, CopG family | Antitoxin |
| LVJ73_RS03500 (LVJ73_03495) | 756997..757434 | - | 438 | WP_003403386.1 | type II toxin-antitoxin system VapC family toxin | - |
| LVJ73_RS03505 (LVJ73_03500) | 757431..757685 | - | 255 | WP_003911263.1 | antitoxin VapB7 | - |
| LVJ73_RS03510 (LVJ73_03505) | 757799..760162 | + | 2364 | WP_003898547.1 | arylsulfatase AtsD | - |
| LVJ73_RS03515 (LVJ73_03510) | 760225..760551 | + | 327 | WP_003403401.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | - |
| LVJ73_RS03520 (LVJ73_03515) | 760463..760801 | + | 339 | WP_003403405.1 | PIN domain-containing protein | - |
| LVJ73_RS03525 (LVJ73_03520) | 760798..760971 | + | 174 | WP_003898549.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 103 a.a. Molecular weight: 11320.10 Da Isoelectric Point: 11.6554
>T229037 WP_003403376.1 NZ_CP089778:c756655-756347 [Mycobacterium tuberculosis]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC
Download Length: 309 bp
>T229037 NZ_CP124374:519414-519791 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA
Antitoxin
Download Length: 82 a.a. Molecular weight: 9169.01 Da Isoelectric Point: 4.2070
>AT229037 WP_003403381.1 NZ_CP089778:c756887-756642 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R
Download Length: 246 bp
>AT229037 NZ_CP124374:518956-519324 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U4BSE5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | G0TQE0 |