Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 754646..755279 Replicon chromosome
Accession NZ_CP089778
Organism Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)


Gene name vapC Uniprot ID G0TQD6
Locus tag LVJ73_RS03475 Protein ID WP_003403365.1
Coordinates 754646..755029 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ56
Locus tag LVJ73_RS03480 Protein ID WP_003403368.1
Coordinates 755124..755279 (-) Length 52 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ73_RS03450 (LVJ73_03445) 749938..750474 + 537 WP_003403341.1 50S ribosomal protein L10 -
LVJ73_RS03455 (LVJ73_03450) 750511..750903 + 393 WP_003403353.1 50S ribosomal protein L7/L12 -
LVJ73_RS03460 (LVJ73_03455) 750896..751591 - 696 WP_003403355.1 TetR/AcrR family transcriptional regulator -
LVJ73_RS03465 (LVJ73_03460) 751662..753167 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
LVJ73_RS03470 (LVJ73_03465) 753248..754258 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
LVJ73_RS03475 (LVJ73_03470) 754646..755029 - 384 WP_003403365.1 ribonuclease VapC6 Toxin
LVJ73_RS03480 (LVJ73_03475) 755124..755279 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ73_RS03485 (LVJ73_03480) 755355..756071 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
LVJ73_RS03490 (LVJ73_03485) 756347..756655 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
LVJ73_RS03495 (LVJ73_03490) 756642..756887 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family -
LVJ73_RS03500 (LVJ73_03495) 756997..757434 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
LVJ73_RS03505 (LVJ73_03500) 757431..757685 - 255 WP_003911263.1 antitoxin VapB7 -
LVJ73_RS03510 (LVJ73_03505) 757799..760162 + 2364 WP_003898547.1 arylsulfatase AtsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Bacterial antitoxin of type II TA system, VapB

(3-44)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13959.73 Da        Isoelectric Point: 6.0803

>T229036 WP_003403365.1 NZ_CP089778:c755029-754646 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Download         Length: 384 bp

>T229036 NZ_CP124374:419803-420153 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 52 a.a.        Molecular weight: 5872.72 Da        Isoelectric Point: 5.7734

>AT229036 WP_003403368.1 NZ_CP089778:c755279-755124 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Download         Length: 156 bp

>AT229036 NZ_CP124374:419558-419809 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQD6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BSF8

References