Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 717539..718188 Replicon chromosome
Accession NZ_CP089778
Organism Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)


Gene name vapC Uniprot ID P67241
Locus tag LVJ73_RS03290 Protein ID WP_003403236.1
Coordinates 717793..718188 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ34
Locus tag LVJ73_RS03285 Protein ID WP_003403235.1
Coordinates 717539..717793 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ73_RS03260 (LVJ73_03255) 712665..712748 + 84 Protein_646 galactose-1-phosphate uridylyltransferase -
LVJ73_RS03265 (LVJ73_03260) 712767..713848 + 1082 Protein_647 galactose-1-phosphate uridylyltransferase -
LVJ73_RS03270 (LVJ73_03265) 713845..714936 + 1092 WP_003906403.1 galactokinase -
LVJ73_RS03275 (LVJ73_03270) 715331..716395 + 1065 WP_003898532.1 zinc ribbon domain-containing protein -
LVJ73_RS03280 (LVJ73_03275) 716499..717446 + 948 WP_003403232.1 DUF732 domain-containing protein -
LVJ73_RS03285 (LVJ73_03280) 717539..717793 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 Antitoxin
LVJ73_RS03290 (LVJ73_03285) 717793..718188 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 Toxin
LVJ73_RS03295 (LVJ73_03290) 718282..719022 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
LVJ73_RS03300 (LVJ73_03295) 719154..719414 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ73_RS03305 (LVJ73_03300) 719411..719818 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin -
LVJ73_RS03310 (LVJ73_03305) 719890..721041 - 1152 WP_003403248.1 FIST N-terminal domain-containing protein -
LVJ73_RS03315 (LVJ73_03310) 721134..722861 - 1728 WP_003900979.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14250.04 Da        Isoelectric Point: 4.7475

>T229034 WP_003403236.1 NZ_CP089778:717793-718188 [Mycobacterium tuberculosis]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Download         Length: 396 bp

>T229034 NZ_CP124373:140346-140762 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9139.52 Da        Isoelectric Point: 9.2702

>AT229034 WP_003403235.1 NZ_CP089778:717539-717793 [Mycobacterium tuberculosis]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Download         Length: 255 bp

>AT229034 NZ_CP124373:140119-140349 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4XGR
PDB 4XGQ

Antitoxin

Source ID Structure
PDB 4XGR
PDB 4XGQ
AlphaFold DB A0A7U4BSC2

References